Fourier-Transform Raman and Infrared Spectroscopy

傅里叶变换拉曼和红外光谱

• 批准号: 9850474
• 负责人: Norman Craig
• 金额: $ 4.83万
• 依托单位: Oberlin College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9850474&HistoricalAwards=false
• 关键词: Fourier; Transform; Raman; Infrared; Spectroscopy

Fourier transform-Raman spectroscopic experiments are being introduced into several laboratories for chemistry and biochemistry majors and other upper-level science students. The higher resolution (about 0.1 cm-l) and near-infrared range of the new FT-Raman instrument permit more sophisticated experiments. For the biochemistry laboratory, students use Raman spectroscopy in a new experiment to study synthetic phospholipid bilayers dispersed in water. Students will monitor the change from ordered to disordered structure in the hydrocarbon chains in the interior of dipalmitoylphosphatidylchloine bilayers as a function of temperature by observing intensities in the CH and CC stretching regions. The effect of cholesterol as an additive is studied allowing students learn more about the structure of cell membranes. In the analytical chemistry laboratory students use Raman spectroscopy to study the composition of a standard xylenes mixture using the method of internal standards. They will also compare the ease of sampling for Raman and infrared spectroscopies. For the physical chemistry laboratory, the scope of analyzing vibration-rotation bands by infrared spectroscopy will be extended. As an alternative to the classic experiment of analyzing the rotational structure due to the two chlorine isotopes in HCl in the gas-phase vibration-rotation absorption band, it is now possible for students to do a similar experiment on the bromine isotopes in HBr. The improved near-infrared capability facilitates the study of overtones in the HCl or HBr spectrum and thus mapping the lower part of the potential well and finding how the bond length varies with vibrational excitation. For students in the advanced analytical and physical laboratory, a new experiment comparing the Raman and infrared spectra of the cis and trans isomers of 1,2-dichloroethylene provides data used to assign all twelve of the vibrational fundamentals of each of the isomers. Both the infrared and Raman features of the new instrument support und ergraduate student research. This instrumentation provides opportunities for students to have a broader, deeper understanding of the theory and application of vibrational spectroscopy.

傅里叶变换拉曼光谱实验正在被引入化学和生物化学专业以及其他高级理科学生的几个实验室。 新型 FT-拉曼仪器具有更高的分辨率（约 0.1 cm-l）和近红外范围，可以进行更复杂的实验。在生物化学实验室中，学生们在一项新实验中使用拉曼光谱来研究分散在水中的合成磷脂双层。学生将通过观察 CH 和 CC 拉伸区域的强度来监测二棕榈酰磷脂酰氯双层内部烃链从有序结构到无序结构的变化，作为温度的函数。研究胆固醇作为添加剂的作用，让学生更多地了解细胞膜的结构。在分析化学实验室中，学生使用拉曼光谱法通过内标方法研究标准二甲苯混合物的组成。他们还将比较拉曼和红外光谱采样的难易程度。对于物理化学实验室，红外光谱分析振动-旋转谱带的范围将得到扩展。作为分析 HCl 中两种氯同位素在气相振动旋转吸收带中的旋转结构的经典实验的替代方案，学生现在可以对 HBr 中的溴同位素进行类似的实验。改进的近红外能力有助于研究 HCl 或 HBr 光谱中的泛音，从而绘制势阱下部的图并发现键长如何随振动激发而变化。对于高级分析和物理实验室的学生来说，一项比较 1,2-二氯乙烯顺式和反式异构体的拉曼光谱和红外光谱的新实验提供了用于分配每种异构体的所有 12 个振动基本原理的数据。新仪器的红外和拉曼功能都支持本科生的研究。该仪器为学生提供了对振动光谱学理论和应用有更广泛、更深入了解的机会。