Fourier transform raman micro-spectroscopy with IR excitation : a unique system to characterize bio-inorganic materials and interfaces

红外激发的傅里叶变换拉曼显微光谱：表征生物无机材料和界面的独特系统

• 批准号: 405949-2011
• 负责人: Cerruti, Marta
• 金额: $ 10.85万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472350
• 关键词: Fourier; transform; raman; micro; spectroscopy

We propose the purchase of an FT-Raman spectroscope with 1064 nm laser excitation to be combined with a Raman microscope present in Cerruti's lab. This combined system does not exist anywhere in Canada, and is essential for the analysis of samples that fluoresce when excited with visible radiation. Raman microscopy is conventionally performed with visible or UV lasers. However, lasers in the visible range often excite fluorescent radiation, which can be overwhelming in biological and organometallic samples. To avoid this problem and still have a large enough signal/noise ratio, Fourier Transform Raman spectroscopy with IR laser excitation was developed. The IR laser does not generate fluorescence in the samples; the radiation collected from the sample goes through an interferometer before hitting the detector, thus allowing the collection of a whole spectrum in less than a second. By accumulating hundreds of spectra the signal/noise ratio is dramatically increased. Here we propose to buy an FT-Raman spectrometer with a laser at 1064 nm, which we will connect to a Raman microscope already present in Cerruti's lab. This combination will allow us to collect FT-Raman spectra using the 1064 nm laser with a spatial resolution of ~1 micron, as opposed to the resolution of ~50 micron obtained with a microscope stage set in the FT-Raman spectrometer. This combination is extremely rare, and no other systems like this exist in Canada. It is a crucial tool to analyze bio-inorganic materials and interfaces, and this system will support the programs of at least seven researchers from Mining and Materials, Chemical, Mechanical, Bio-Engineering, and Chemistry at McGill and at least 46 of their trainees. We expect many more users will be attracted once the system is installed, for example among members of the McGill Institute for Advanced Materials, the Center for Bone and Periodontal Disease, and the Center for Biorecognition and Biosensors. Working with FT-Raman spectro-microscopy, the students will extend their expertise in materials surface characterization, especially for applications in biomaterials, bioengineering, biosensing, pharmaceutics, forensic, and art restoration.

我们建议购买一台1064nm激光激发的ft -拉曼光谱仪，与Cerruti实验室的拉曼显微镜结合使用。这种组合系统在加拿大的任何地方都不存在，对于用可见辐射激发发出荧光的样品的分析是必不可少的。拉曼显微镜通常是用可见光或紫外激光进行的。然而，可见光范围内的激光经常激发荧光辐射，这在生物和有机金属样品中可能是压倒性的。为了避免这一问题，同时保证足够大的信噪比，研究了红外激光激发的傅里叶变换拉曼光谱。红外激光在样品中不产生荧光；从样品中收集的辐射在到达探测器之前经过干涉仪，因此可以在不到一秒的时间内收集整个光谱。通过累积数百个光谱，显著提高了信噪比。我们建议购买一台激光波长为1064nm的FT-Raman光谱仪，并将其与Cerruti实验室已有的Raman显微镜连接。这种组合将使我们能够使用1064 nm激光器以~1微米的空间分辨率收集ft -拉曼光谱，而不是ft -拉曼光谱仪中设置的显微镜平台获得的~50微米的分辨率。这种组合非常罕见，在加拿大没有其他类似的系统存在。它是分析生物无机材料和界面的关键工具，该系统将支持麦吉尔大学采矿与材料、化学、机械、生物工程和化学领域至少7名研究人员和至少46名受训人员的项目。我们预计，一旦系统安装，将吸引更多的用户，例如麦吉尔先进材料研究所、骨骼和牙周病中心、生物识别和生物传感器中心的成员。利用ft -拉曼光谱显微镜，学生将扩展他们在材料表面表征方面的专业知识，特别是在生物材料、生物工程、生物传感、制药、法医和艺术修复方面的应用。