Molecular, genetic and phenotypic analysis of genes controlling lateral spikelet fertility in barley

控制大麦侧小穗育性的基因的分子、遗传和表型分析

• 批准号: 130726083
• 负责人: Professor Dr. Thorsten Schnurbusch
• 金额: --
• 依托单位: Abteilung Züchtungsforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/130726083?language=en
• 关键词: Molecular; genetic; phenotypic; analysis; genes

Our understanding of the developmental genetics of grass inflorescences is very limited. Functional knowledge of genes which regulate key morphological and developmental traits such as spikelet initiation, abortion, or fertility is almost completely lacking in most cereal crops. Hence, within the scope of this project we will focus on the molecular elucidation of lateral spikelet fertility in barley. To this end, we will investigate three mutant lines—vrs2, vrs3, and, vrs4—which display almost complete lateral spikelet fertility (six-rowed phenotype) compared with known six-rowed genotypes (vrs1). During the proposed project we will (i) perform detailed phenotypic analyses and molecular mapping of six-rowed mutants, (ii) identify and isolate novel grass- and/or species-specific genes/proteins which control lateral spikelet fertility, and (iii) study the expression pattern, tissue-specificity and function of gene(s) during spike development. The molecular isolation of genes involved in lateral spikelet fertility will make an important contribution to future increases in cereal grain yields by providing further insights into the developmental genetic processes underlying spike morphology in barley and other grasses.

我们对禾本科植物花序发育遗传学的理解是非常有限的。在大多数谷类作物中，几乎完全缺乏调控小穗起始、败育或育性等关键形态和发育性状的基因的功能知识。因此，在这个项目的范围内，我们将集中在大麦侧小穗育性的分子阐明。为此，我们将调查三个突变株系vrs 2，vrs 3，和vrs 4-显示几乎完全侧小穗育性（六棱表型）相比，已知的六棱基因型（vrs 1）。在拟议的项目中，我们将（i）进行详细的表型分析和六棱突变体的分子定位，（ii）确定和分离新的草和/或物种特异性基因/蛋白质控制侧小穗育性，和（iii）研究在穗发育过程中的基因的表达模式，组织特异性和功能。参与侧小穗育性的基因的分子分离将通过进一步了解大麦和其他禾本科植物穗形态的发育遗传过程，为未来谷物产量的增加做出重要贡献。

项目成果

Six-rowed spike4 (Vrs4) controls spikelet determinacy and row-type in barley

• DOI: 10.1073/pnas.1221950110
• 发表时间: 2013-08-06
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Koppolu, Ravi;Anwar, Nadia;Schnurbusch, Thorsten
• 通讯作者: Schnurbusch, Thorsten