SGER: Discovering Novel Bacterial Metabolic Genes using Differential Display

需要一种直接的方法来鉴定在混合培养或环境中表达的新基因。 本SGER的目的是调整和验证差异显示（DD）方法（Liang和Pardee，1992），该方法最初是为真核系统开发的，可用于微生物生物学研究。 最近设计的一种基于E.大肠杆菌编码序列（Fislage等，1996）将使用其他变形菌和一氧化二氮还原酶（nos）基因作为靶标进行测试。 或者，将使用Genbank和其他储存库中可用的各种真细菌的完整基因组设计用于DD的新引发组。 这项研究代表了在财团或环境中发现传统方法无法检测到的新基因的唯一方法之一。

KerkhofA direct way of identifying novel genes being expressed in mixed culture or the environment is needed. The goal of this SGER is to adapt and verify the differential display (DD) method (Liang and Pardee, 1992), originally developed for eukaryotic systems, for use in microbial biology research. A recently designed priming set based on E. coli coding sequence (Fislage et al., 1996) will be tested using other Proteobacteria and the nitrous oxide reductase (nos) gene as a target. Alternatively, a new priming set for DD will be designed using the complete genomes of various eubacteria available in Genbank and other repositories. This research represents one of the only ways to discover novel genes in consortia or the environment that can not be detected using traditional approaches.