Thermodynamics and Kinetics of Protein Aggregations

蛋白质聚集体的热力学和动力学

• 批准号: 9901054
• 负责人: Harvey Blanch
• 金额: $ 39.87万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9901054&HistoricalAwards=false
• 关键词: Thermodynamics; Kinetics; Protein; Aggregations

The objective of this project is to use a combination of experiment and theory/simulations to study protein aggregation as a competitive process to folding at high protein concentrations. One strategy will be to study, by experiment, the influence of specific interactions between amino acid residues and secondary structure on the stability of aggregates vs. the native three-dimensional structure. A secondary strategy will be to determine, from computer simulations and theory, the general characteristics of minimal models of heterogeneous polymers that can either aggregate or fold.

该项目的目的是使用实验和理论/模拟相结合的方法来研究蛋白质聚集作为高蛋白质浓度下折叠的竞争过程。一个策略将是通过实验研究氨基酸残基和二级结构之间的特定相互作用对聚集体稳定性的影响，而不是天然的三维结构。第二种策略是通过计算机模拟和理论来确定既可以聚集也可以折叠的非均相聚合物的最小模型的一般特征。