Molecular Thermodynamics of Protein Precipitation in AqueousSolution

水溶液中蛋白质沉淀的分子热力学

• 批准号: 8914849
• 负责人: Harvey Blanch
• 金额: $ 30.5万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-15 至 1993-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8914849&HistoricalAwards=false
• 关键词: Molecular; Thermodynamics; Protein; Precipitation; AqueousSolution

The proposed research is concerned with separating a mixture of proteins in aqueous solution through selective precipitation, i. e., by formation of a phase rich in the target protein in equilibrium with a second phase where the concentration of the target protein is very low. Precipitation is achieved by salts or nonionic polymers or both. To obtain a quantitative understanding of protein- precipitation equilibria, a molecular-thermodynamic model based on an extended Guggenheim/McMillan-Mayer osmotic viral expansion is presented. The goal of this research is to establish an engineering-oriented correlation for rational design of protein-precipitation processes. A long-range benefit of this research is that it is likely to provide useful knowledge for better understanding of protein crystallization.

拟议的研究涉及分离混合物 蛋白质在水溶液中通过选择性 降水量岛即，通过形成富含 靶蛋白与第二相平衡，其中 靶蛋白的浓度非常低。 沉淀是通过盐或非离子聚合物或 两者 为了对蛋白质有一个定量的了解- 沉淀平衡，分子热力学模型 基于古根海姆/麦克米兰-迈耶渗透性病毒 提出了扩展。 本研究的目标是 建立一个面向工程关联， 蛋白质沉淀过程的设计。 一枚远程 这项研究的好处是，它很可能提供 有用的知识，以更好地了解蛋白质 晶化