Engineering Protein Aggregation and Fibril Formation

本研究的目的是研究初级序列和构象特征在决定蛋白质聚集行为中的作用。具体目标是针对潜在机制的实验和理论研究。最终目标是控制聚合。为了帮助揭示操纵蛋白质聚集特性所必需的力量和机制，建议的研究重点是序列模式和溶液参数对聚集和重折叠之间竞争的影响。实验工作将在多个感兴趣的系统上进行，包括短寡肽以及三种不同蛋白质的特定区域，所有这些都是研究聚集的合适候选者。

0432625BlanchThe goal of this proposed research is investigate the roles of primary sequence and conformational characteristics in determining protein aggregation behavior. The specific objectives are directed toward experimental and theoretical investigations of the underlying mechanisms. The ultimate goal is to control aggregation. To assist in uncovering forces and mechanisms essential to manipulate protein aggregation properties, the proposed research focuses on the effects of sequence patterns and solution parameters that impact the competition between aggregation and refolding. The experimental work will be performed on multiple systems of interest, including short oligopeptides as well as specific regions of three different proteins, all of which are suitable candidates for studies of aggregation.