Engineering Protein Aggregation and Fibril Formation

工程蛋白质聚集和原纤维形成

• 批准号: 0432625
• 负责人: Harvey Blanch
• 金额: $ 49.92万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-15 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0432625&HistoricalAwards=false
• 关键词: Engineering; Protein; Aggregation; Fibril; Formation

0432625BlanchThe goal of this proposed research is investigate the roles of primary sequence and conformational characteristics in determining protein aggregation behavior. The specific objectives are directed toward experimental and theoretical investigations of the underlying mechanisms. The ultimate goal is to control aggregation. To assist in uncovering forces and mechanisms essential to manipulate protein aggregation properties, the proposed research focuses on the effects of sequence patterns and solution parameters that impact the competition between aggregation and refolding. The experimental work will be performed on multiple systems of interest, including short oligopeptides as well as specific regions of three different proteins, all of which are suitable candidates for studies of aggregation.

0432625 Blanch本研究的目的是研究一级序列和构象特征在决定蛋白质聚集行为中的作用。 具体目标是针对实验和理论研究的基本机制。 最终目标是控制聚集。 为了帮助揭示操纵蛋白质聚集特性所必需的力量和机制，拟议的研究集中在影响聚集和重折叠之间竞争的序列模式和溶液参数的影响上。 实验工作将在多个感兴趣的系统上进行，包括短寡肽以及三种不同蛋白质的特定区域，所有这些都是聚集研究的合适候选者。