The Role of Gene Duplication in the Evolution of Chordate Developmental Regulation

基因复制在脊索动物发育调控进化中的作用

• 批准号: 9905403
• 负责人: Frank Ruddle
• 金额: $ 49.71万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-10-01 至 2002-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9905403&HistoricalAwards=false
• 关键词: Role; Gene; Duplication; Evolution; Chordate

This collaborative project between the laboratories at Yale (Ruddle and Wagner) and Boston University (Amemiya) seeks to understand the evolutionary diversification of the vertebrate body plan in molecular and developmental terms. Key organisms such as the lamprey, horned shark, and the primitive bony fish Bichir will be major subjects for research. A primary approach to the problem of the vertebrate radiation will be to isolate and sequence the Hox gene clusters that govern pattern formation on the primary and secondary body axes. In particular, the investigators will be interested in determining copy number of the clusters in the selected species, the identification of conserved non-coding elements that may serve as regulatory elements, the history of Hox cluster duplications and their possible role in vertebrate evolution. Finally, the investigators want to test the neutralist theory with respect to the duplication and diversification of gene complexes governing body plan.

耶鲁大学（Ruddle和瓦格纳）和波士顿大学（Amemiya）实验室之间的这个合作项目旨在从分子和发育方面了解脊椎动物身体计划的进化多样性。 七鳃鳗、角鲨、原始硬骨鱼Bichir等重要生物将是主要研究对象。 解决脊椎动物辐射问题的主要方法是分离和测序控制初级和次级体轴图案形成的Hox基因簇。 特别是，研究人员将有兴趣确定所选物种中簇的拷贝数，鉴定可能作为调控元件的保守非编码元件，Hox簇复制的历史及其在脊椎动物进化中的可能作用。 最后，研究者希望检验关于基因复合体管理体计划的重复和多样化的中立主义理论。