Preparation and high-throughput screening of integrated bioelectrocatalytic redox polymeric systems to provide a toolbox for development of electrochemical devices for sensor and energy conversion

集成生物电催化氧化还原聚合物系统的制备和高通量筛选，为传感器和能量转换电化学装置的开发提供工具箱

• 批准号: 133775033
• 负责人: Professor Dr. Wolfgang Schuhmann
• 金额: --
• 依托单位: School of Biological and Chemical Sciences
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/133775033?language=en
• 关键词: Preparation; throughput; screening; integrated; bioelectrocatalytic

A rational, designed route for the preparation of materials, allowing variation in biomolecule source, electronic connection through redox mediation and delivery of resilient, stable materials through immobilization, coupling chemistry and surface engineering is proposed. Synthesis of transition metal complex libraries with rational designed coordination sphere and formal potential on the one hand and the sourcing of functionalized polymers, and parallel synthesis of libraries of electrodeposition polymers (EDP), with predefined properties is the basis for the development of large libraries of redox polymers. Integration of enzymes within these redox polymers enables the design of electron-transfer pathways between the polymer-immobilized biological recognition elements and an electrode surface as a basis for the development of biosensors and especially biofuel cell electrodes. The parallel synthesis approach in combination with a high-throughput approach for both characterization of the individual members of the polymer library as well as the automated fabrication and testing of sensor configurations and biofuel cells will provide insights into the complex set of parameters which are interacting and determining the properties of amperometric biosensors and the features of biofuel cells. The research programme proposed will establish the building blocks for such a combined approach, to include the targeted synthesis and characterization of a library of mediating redox complexes and polymerizable supports, co-immobilization methodologies of these complexes with suitable biological elements, characterization of the films and surfaces, and investigation of their applications as biomolecular electronic devices such as (bio)sensing and (bio)fuel cells.

提出了一种合理的、设计的材料制备路线，允许生物分子来源的变化、通过氧化还原介导的电子连接以及通过固定化、偶联化学和表面工程递送弹性、稳定的材料。一方面，具有合理设计的配位球和形式电势的过渡金属络合物库的合成以及官能化聚合物的来源，以及具有预定义性质的电沉积聚合物（EDP）库的平行合成是开发大型氧化还原聚合物库的基础。这些氧化还原聚合物内的酶的整合使得能够设计聚合物固定的生物识别元件和电极表面之间的电子转移路径，作为生物传感器，特别是生物燃料电池电极的开发的基础。并行合成方法与高通量方法相结合，用于表征聚合物库的各个成员以及传感器配置和生物燃料电池的自动化制造和测试，将提供对复杂参数集的深入了解，这些参数集相互作用并确定电流型生物传感器的特性和生物燃料电池的特征。拟议的研究计划将为这种组合方法建立基石，包括介导氧化还原复合物和可聚合载体库的有针对性的合成和表征、这些复合物与合适生物元素的共固定方法、薄膜和表面的表征，以及对其作为生物分子电子设备（例如（生物）传感和（生物）燃料电池）的应用的研究。

项目成果

A low-potential glucose biofuel cell anode based on a toluidine blue modified redox polymer and the flavodehydrogenase domain of cellobiose dehydrogenase

• DOI: 10.1016/j.elecom.2013.01.016
• 发表时间: 2013-04
• 期刊: Electrochemistry Communications
• 影响因子: 5.4
• 作者: Minling Shao;Sascha Pöller;C. Sygmund;R. Ludwig;W. Schuhmann

A new synthesis route for Os-complex modified redox polymers for potential biofuel cell applications.

用于潜在生物燃料电池应用的Os复合物改性氧化还原聚合物的新合成路线

• DOI: 10.1016/j.bioelechem.2011.11.015
• 发表时间: 2012
• 期刊: Bioelectrochemistry
• 影响因子: 5
• 作者: S. Pöller;Y. Beyl;J. Vivekananthan;D. A. Guschin;W. Schuhmann
• 通讯作者: W. Schuhmann

Coupling osmium complexes to epoxy-functionalised polymers to provide mediated enzyme electrodes for glucose oxidation.

• DOI: 10.1016/j.bios.2012.11.036
• 发表时间: 2013-05
• 期刊: Biosensors & bioelectronics
• 影响因子: 12.6
• 作者: Peter Ó Conghaile;Sascha Pöller;Domhnall MacAodha;W. Schuhmann;D. Leech

NADH oxidation using modified electrodes based on lactate and glucose dehydrogenase entrapped between an electrocatalyst film and redox catalyst-modified polymers

• DOI: 10.1007/s00604-012-0797-2
• 发表时间: 2012-06-01
• 期刊: MICROCHIMICA ACTA
• 影响因子: 5.7
• 作者: Al-Jawadi, Eman;Poeller, Sascha;Schuhmann, Wolfgang
• 通讯作者: Schuhmann, Wolfgang

Dehydrogenase‐Based Reagentless Biosensors: Electrochemically Assisted Deposition of Sol‐Gel Thin Films on Functionalized Carbon Nanotubes

基于脱氢酶的无试剂生物传感器：在功能化碳纳米管上电化学辅助沉积 SolâGel 薄膜

• DOI: 10.1002/elan.201100574
• 发表时间: 2012
• 期刊: Electroanalysis
• 影响因子: 3
• 作者: Z. Wang;M. Etienne;S. Pöller;W. Schuhmann;G.-W. Kohring;V. Mamane;A. Walcarius
• 通讯作者: A. Walcarius