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Graphene-based optoelectrochemical sensor for the simultaneous monitoring of the electrical and chemical activity of single cells

基于石墨烯的光电化学传感器，用于同时监测单细胞的电学和化学活性

基本信息

批准号：
279144839
负责人：
Professor Dr. Wolfgang Schuhmann
金额：
--
依托单位：
Institut dÉlectronique de Microélectronique et de Nanotechnologie (IEMN)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2015
资助国家：
德国
起止时间：
2014-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/279144839?language=en
关键词：
Graphene based optoelectrochemical sensor simultaneous

项目摘要

Improved understanding of molecular principles of neuronal communication allows new insights into neurodegenerative diseases and possibly to new therapeutic targets. However, there is a lack of tools able to monitor simultaneously electrical and chemicals signals of single cells. Therefore, we propose the development of a novel graphene-enhanced sensor allowing simultaneous monitoring of these two signals with single cell resolution both in cell cultures and organotypic tissue slices. The sensor will be based on surface plasmon resonance imaging (SPRi) and its ability to provide excellent spatial resolution also to electrochemical measurements. More importantly, by integrating graphene both the sensitivity of SPR detection and the current densities of the electrochemical measurement will be enhanced with concomitantly enhanced biocompatibility. In addition to generating new knowledge about the interplay of electrical and chemical signals of living cells, the development of the anticipated sensors will be an important step towards novel prostheses based on the bidirectional communication with living cells. The core of the sensor will be a cell chip carrying disk microelectrodes, to which cells adhere, surrounded by cell-free ring microelectrodes. Once cells have adhered at the disk microelectrodes, the ring microelectrodes (modified with enzymes or unmodified) are polarized to a potential that allows oxidation or reduction of signaling molecules secreted by the cells. Subsequently, high-resolution SPR images of the cell chip are recorded at high frame rates (~ 10000 fps) while a physical or chemical stimulus is applied to the cells. SPR images of cell-covered disk microelectrodes are modulated by changes in the extracellular field potential of the cells which e.g. enables to monitor the propagation of action potentials. SPR images of the ring microelectrodes will be altered by changes in local current densities invoked by variations in the local concentrations of signaling molecules and will be used to observe chemical signals from the cells. Neuronal cells change their extracellular field potential within the low millivolts range and release only tiny amounts of signaling molecules. Therefore, the sensitivity of SPRi has to be improved in order to be able to record electrical and chemical signals from cells simultaneously. Graphene has already been proved to enhance the sensitivity of both SPR and electrochemical detection. Hence, graphene will be investigated for signal amplification. Our approach to measure extracellular field potentials will replace the application of voltage sensitive dyes while it will at least maintain the spatial resolution of optical microscopy which is superior to the spatial resolution provided by the electrically interrogated microelectrode array. The approach will additionally provide unprecedented spatial resolution and interference elimination to electrochemical monitoring of chemical signals from cells.

对神经元通讯分子原理的深入了解可以为神经退行性疾病提供新的见解，并可能发现新的治疗靶点。然而，缺乏能够同时监测单个细胞的电信号和化学信号的工具。因此，我们提出了一种新型的石墨烯增强传感器的发展，允许同时监测这两个信号与细胞培养和器官型组织切片中的单细胞分辨率。该传感器将基于表面等离子体共振成像（SPRi）及其为电化学测量提供出色空间分辨率的能力。更重要的是，通过集成石墨烯，SPR检测的灵敏度和电化学测量的电流密度都将增强，同时增强生物相容性。除了产生有关活细胞电信号和化学信号相互作用的新知识外，预期传感器的开发将是基于与活细胞双向通信的新型假肢的重要一步。该传感器的核心将是一个细胞芯片携带盘微电极，细胞粘附，周围无细胞的环形微电极。一旦细胞粘附在圆盘微电极上，将环形微电极（用酶修饰或未修饰）极化至允许细胞分泌的信号分子氧化或还原的电位。随后，在对细胞施加物理或化学刺激的同时，以高帧速率（~ 10000 fps）记录细胞芯片的高分辨率SPR图像。细胞覆盖的圆盘微电极的SPR图像通过细胞的胞外场电位的变化来调制，这例如使得能够监测动作电位的传播。环形微电极的SPR图像将通过信号分子局部浓度变化引起的局部电流密度变化而改变，并将用于观察来自细胞的化学信号。神经元细胞在低毫伏范围内改变其胞外场电位，并且仅释放极少量的信号分子。因此，必须提高SPRi的灵敏度，以便能够同时记录来自细胞的电信号和化学信号。石墨烯已经被证明可以提高SPR和电化学检测的灵敏度。因此，石墨烯将被研究用于信号放大。我们的方法来测量细胞外场电位将取代电压敏感染料的应用，同时它将至少保持光学显微镜的空间分辨率，这是上级的空间分辨率提供的电询问微电极阵列。该方法还将为来自细胞的化学信号的电化学监测提供前所未有的空间分辨率和干扰消除。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Sensitive electrochemical detection of cardiac troponin I in serum and saliva by nitrogen-doped porous reduced graphene oxide electrode

DOI：
10.1016/j.snb.2018.01.215
发表时间：
2018-06-01
期刊：
SENSORS AND ACTUATORS B-CHEMICAL
影响因子：
8.4
作者：
Chekin, Fereshteh;Vasilescu, Alina;Szunerits, Sabine
通讯作者：
Szunerits, Sabine

Dopamine-functionalized cyclodextrins: modification of reduced graphene oxide based electrodes and sensing of folic acid in human serum

DOI：
10.1007/s00216-019-01892-1
发表时间：
2019-08-01
期刊：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
影响因子：
4.3
作者：
Chekin, Fereshteh;Mishyn, Vladyslav;Szunerits, Sabine
通讯作者：
Szunerits, Sabine

Porous reduced graphene oxide modified electrodes for the analysis of protein aggregation. Part 1: Lysozyme aggregation at pH 2 and 7.4

DOI：
10.1016/j.electacta.2017.09.083
发表时间：
2017-11
期刊：
Electrochimica Acta
影响因子：
6.6
作者：
A. Vasilescu;Samia Boulahneche;F. Chekin;S. Gáspár;M. Medjram;A. Diagne;Santosh K. Singh;Sreekumar Kurungot;R. Boukherroub;S. Szunerits
通讯作者：
A. Vasilescu;Samia Boulahneche;F. Chekin;S. Gáspár;M. Medjram;A. Diagne;Santosh K. Singh;Sreekumar Kurungot;R. Boukherroub;S. Szunerits

Graphene-modified electrodes for sensing doxorubicin hydrochloride in human plasma