Japan STA Fellowship: Chitin Perception in the Model Legume Lotus japonicus: A Molecular and Biochemical Comparison to a Chitin Binding Protein in Rice

日本 STA 奖学金：豆科植物莲花模型中的几丁质感知：与水稻中几丁质结合蛋白的分子和生化比较

• 批准号: 9907799
• 负责人: Robert Day
• 金额: $ 0.41万
• 依托单位: Fellowships
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9907799&HistoricalAwards=false
• 关键词: Japan; STA; Fellowship; Chitin; Perception

9907799DayThe proposed research by Dr. Robert Bradley Day will examine several questions regarding chitin perception in plants. Clearly, chitin as a chemical signal molecule and elicitor of a number of responses in both plants and animals has begun to emerge in recent years as a candidate molecule for studying gene activation and expression following delivery of an external stimulus. The major hypothesis to be tested by Dr. Day is that chitin perception by rice in response to fungal pathogenesis (a harmful relationship) shares a common recognition event with chitin perception in the Rhizobium-legume symbiosis (a beneficial relationship). At a technical level, the identification of a chitin-binding protein in the model legume Lotus japonicus which is homologous to that identified in rice will lead to a better understanding of chitin perception in general, and of its role in cellular interactions. This award supports a two-year Science and Technology Agency of Japan (STA) Postdoctoral Fellowship for Dr. Robert Bradley Day at the National Institute of Agrobiological Resources, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Japan. Dr. Day will work with Dr. Naoto Shibuya, Director of the Department of Biotechnology, on a project entitled, "Chitin Perception in the Model Legume Lotus japonicus: A Molecular and Biochemical Comparison to a Chitin-Binding Protein in Rice." The experiments in the proposed project will contribute to an understanding of the role of chitin perception, and to a better understanding of the mechanism of signal exchange and the structure-function specificity of this which appears to dictate this complex interaction. There are clear long-term practical applications to agriculture of the findings from this basic research work. The proposed work will benefit through collaboration with the Japanese host laboratory, where Dr. Day will have access to the necessary experimental systems, equipment and expertise.***

罗伯特布拉德利戴博士提出的研究将研究几个关于植物中几丁质感知的问题。显然，甲壳素作为一种化学信号分子和许多反应在植物和动物的激发子已经开始出现在最近几年作为一个候选分子研究基因激活和表达后的外部刺激。戴博士要检验的主要假设是，水稻对真菌致病性的几丁质感知（一种有害的关系）与根瘤菌-豆科植物共生中的几丁质感知（一种有益的关系）有着共同的识别事件。在技术层面上，在模式豆科植物百脉根中鉴定出与水稻中鉴定出的蛋白同源的几丁质结合蛋白将导致更好地理解几丁质的一般感知及其在细胞相互作用中的作用。该奖项支持为期两年的日本科学技术厅（STA）博士后研究金博士罗伯特布拉德利日在国家农业生物资源研究所，农林水产省，筑波，日本。戴博士将与生物技术系主任涩谷直人博士合作，开展一个名为“模式豆类莲藕中的几丁质感知：与水稻中几丁质结合蛋白的分子和生物化学比较”的项目。“拟议项目中的实验将有助于理解几丁质感知的作用，并更好地理解信号交换机制及其结构-功能特异性，这似乎决定了这种复杂的相互作用。这项基础研究工作的发现对农业有明显的长期实际应用。拟议的工作将受益于与日本东道实验室的合作，Day博士将在那里获得必要的实验系统、设备和专业知识。