SGER: Development of Optical Biosensors for Marine Microbial Processes Studies

SGER：用于海洋微生物过程研究的光学生物传感器的开发

• 批准号: 9907983
• 负责人: Paul Kemp
• 金额: $ 7.76万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9907983&HistoricalAwards=false
• 关键词: SGER; Development; Optical; Biosensors; Marine

Proposal 99-07983 KempThe proposed instrumentation project requests funds to begin development an optical biosensor system for marine microbial population and process studies. The biosensor system will employ fluorescence detection to measure the hybridization of nucleic acids to probe molecules bound to the surface of an optical fiber. The fiber-optic system will be used to assay for the abundance of specific genes, the expression of those genes, or the physiological status of cells within mixed microbial communities. Samples from seawater and marine sediments will be analyzed in the lab by extracting nucleic acids and assaying for one or more nucleic acid targets. Ultimately, the PI's seek the capacity for automated measurements of bacterial dynamics, activity and gene expression in situ.

提议的仪器项目要求资金开始开发用于海洋微生物种群和过程研究的光学生物传感器系统。该生物传感器系统将采用荧光检测来测量核酸的杂交，以探测与光纤表面结合的分子。光纤系统将用于测定特定基因的丰度，这些基因的表达，或混合微生物群落中细胞的生理状态。海水和海洋沉积物样品将在实验室中通过提取核酸和分析一个或多个核酸目标进行分析。最终，PI寻求自动测量细菌动力学、活性和基因原位表达的能力。