Production of a Recombinant Improved Bacillus stearothermophilus DNA Polymerase for DASL Isothermal Amplification

用于 DASL 等温扩增的重组改良嗜热脂肪芽孢杆菌 DNA 聚合酶的生产

• 批准号: 531095-2018
• 负责人: Williamson, Vanessa
• 金额: $ 1.82万
• 依托单位: Seneca College of Applied Arts and Technology
• 依托单位国家: 加拿大
• 项目类别: Applied Research and Development Grants - Level 1
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=668641
• 关键词: Production; Recombinant; Improved; Bacillus; stearothermophilus

Custom Biologics, an established developer of bioanalytical assays, has in-licensed a patented, isothermal, nucleic acid amplification and detection technology from GeneBio Systems Inc. The technology, known as DASL RAPID, is a rapid, sensitive and specific molecular test platform requiring the use of DNA polymerase enzymes. Although effective, the generic DNA polymerase enzymes currently used to support DASL RAPID reactions are costly and perform sub-optimally, which limits the resulting products' commercial use. The objective of this collaboration with Seneca is to research and develop, utilizing protein engineering and recombinant expression technology, a more efficient Bacillus stearothermophilus (Bst) DNA polymerase, to replace the expensive, inefficient, generic enzyme currently used in Custom Biologic's DASL RAPID tests. The result will be an optimized technology platform, which will increase test speed, sensitivity and cost profile. Dr. Frank Merante will lead the project, supported by student Research Assistants from Seneca's School of Biological Science and Applied Chemistry (BSAC), who will apply their academic knowledge and skills, while developing practical experience in biotechnology and protein engineering, thereby enhancing their competitiveness as they transition from academia to industry.

Custom Biologics是一位既定的生物分析测定开发商，它已获得了Genebio Systems Inc.的专利，等温，核酸扩增和检测技术的许可。该技术（称为DASL Rapid）是一个快速，敏感和特定的分子测试平台，需要使用DNA聚合酶enzymes。尽管有效，但目前用于支持DASL快速反应的通用DNA聚合酶酶是昂贵的，并且在次优的情况下执行，这限制了所得产品的商业用途。与塞内卡（Seneca）合作的目的是研究和开发，利用蛋白质工程和重组表达技术，一种更有效的芽孢杆菌硬质嗜热（BST）DNA聚合酶，以取代当前在定制生物学的DASL快速测试中使用的昂贵，效率低下的通用酶。结果将是一个优化的技术平台，该平台将提高测试速度，灵敏度和成本概况。弗兰克·梅兰特（Frank Merante）博士将领导该项目，并得到了塞内卡（Seneca）生物科学和应用化学学院（BSAC）的学生研究助理的支持，他们将运用他们的学术知识和技能，同时发展生物技术和蛋白质工程方面的实践经验，从而在从学术界转变为行业时增强了竞争力。