High Field NMR Instrumentation for Structural Studies of Proteins and Nucleic Acids

9970232 David P.GiedrocTexas A&amp；M University用于蛋白质和核酸结构研究的高场核磁共振仪器这笔赠款支持在德克萨斯A&Amp；M生物化学和生物物理系购买一台600 MHz的核磁共振光谱仪，该光谱仪将大大加强德克萨斯A&Amp对蛋白质和核酸结构和动力学的研究。将受益于新光谱仪的项目包括：(1)调节基因表达的蛋白质的结构和动力学的研究，(2)与艾滋病毒相关的趋化因子的结构和动力学的研究，(3)关于催化RNA分子的结构、热力学和金属离子结合的研究，(4)侧重于蛋白质和核酸中氢键的研究，(5)蛋白质的从头设计和表征，以及(6)含有模拟蛋白质中发现的转折的元素的肽的构象分析。这些研究都不能使用德克萨斯A&M研究人员目前可用的光谱仪来完成，除了对德克萨斯A&M的研究产生重大影响外，600 MHz光谱仪预计还将对学生的培训产生影响。新的光谱仪将成为生物分子核磁共振设施的一部分。该设施为研究生、研究生和本科生提供使用高场核磁共振设备的机会。来自三个不同系的学生使用这个设施。增加一台600 MHz光谱仪将显著扩大学生在大分子结构和动力学领域的培训。

ABSTRACT9970232David P. GiedrocTexas A&M UniversityHigh Field NMR Instrumentation for Structural Studies of Proteins and Nucleic AcidsThis grant supports the acquisition of a 600 MHz NMR spectrometer in the Department of Biochemistry and Biophysics at Texas A&M. The spectrometer will significantly enhance the study of protein and nucleic acid structure and dynamics at Texas A&M. Projects that will benefit from the new spectrometer include: (1) investigation of the structure and dynamics of proteins that regulate gene expression, (2) investigation of the structure and dynamics of chemokines that are related to HIV, (3) studies concerning the structure, thermodynamics, and metal ion binding of catalytic RNA molecules, (4) studies focused on hydrogen bonds in proteins and nucleic acids, (5) de novo design and characterization of proteins, and (6) the analysis of conformations of peptides containing elements that mimic the turns found in proteins. None of these studies can be accomplished using the spectrometers currently available to researchers at Texas A&M.In addition to having a significant impact on research at Texas A&M, the 600 MHz spectrometer is also expected to have an impact on training students. The new spectrometer will become part of the Biomolecular NMR facility. This facility provides postgraduate, graduate, and undergraduate students with access to high field NMR equipment. Students from three different departments use this facility. The addition of a 600 MHz spectrometer will significantly expand the training of students in the area of macromolecular structure and dynamics.