Acquisition of a 500 MHz NMR Spectrometer

小行星9217413 主要研究人员的集体研究兴趣涉及肽和蛋白质结构和分子识别的研究。 将确定与核酸相互作用的两种基因调节锌金属蛋白（锌指蛋白）的金属配位模式和整体结构。 结合底物的构象研究和结构的变化，导致人胰岛素受体的酪氨酸激酶结构域的自磷酸化，提出了研究这类酶的催化。 核磁共振结构研究的有核-片层和模型-三明治蛋白质的计划，以了解的基本规则，管理的形成-片层二级结构的蛋白质。 最后，对生物活性肽的合成构象约束肽模拟物的结构研究，例如， 提出了阿片拮抗剂和活性位点肽作为病毒蛋白酶抑制剂的候选物。 要求核磁共振仪器将在得克萨斯州A M大学生物科学与技术研究所的高分子设计中心的一个组成部分。 由于该中心的主要目标是招募，支持和优秀的本科生，研究生和研究生的教学，拟议的仪器将用于增强和扩大他们在蛋白质结构领域的教育经验。

9217413 Giedroc The collective research interests of the primary investigators concern the study of peptide and protein structure and molecular recognition. The mode of metal coordination and overall structure of two gene regulatory zinc metalloproteins (zinc-finger proteins) which interact with nucleic acids will be determined. Conformational studies of bound substrates and structural changes which result in autophosphorylation of the tyrosine kinase domain of the human insulin receptor are proposed to study catalysis by this class of enzymes. NMR structural studies of nucleated -sheets and model -sandwich proteins are planned to understand the fundamental rules that govern the formation of -sheet secondary structures in proteins. Finally, structural studies of synthetic conformationally constrained peptidomimetics of bio-active peptides, e.g., opiate antagonists and active site peptides as candidates for viral protease inhibitors, are proposed. The request NMR instrumentation will be a component of the Center for Macromolecular Design of the Institute of Biosciences and Technology at Texas A&M University. As a major goal of the Center is the recruitment, support, and teaching of outstanding undergraduate, graduate, and postgraduate students, the proposed instrumentation will be used to enhance and expand their educational experience in the area of protein structure.