Acquisition of a Fluorescence Activated Cell Sorter (FACS)

获取荧光激活细胞分选仪 (FACS)

• 批准号: 9977224
• 负责人: Christopher Hughes
• 金额: $ 30.36万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-15 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9977224&HistoricalAwards=false
• 关键词: Acquisition; Fluorescence; Activated; Cell; Sorter

Acquisition of a Fluorescence Activated Cell Sorter (FACS)Abstract - 9977224One of the most powerful approaches in biology is reductionism, the process of break-ing down complex systems into their component parts, the better to understand their interactions in the whole. A FACS machine allows for the enrichment of specific sub-populations of cells from large, diverse starting populations based on their expression of specific "markers". These surface markers are detected by binding of fluorescently-labeled specific antibodies that glow when exposed to the UV laser in the FACS ma-chine. A diverse population of researchers at UCI has an equally diverse range of interests. These interests can be grouped into four broad areas. These represent just some of the many research and teaching projects on campus that will benefit from a FACS machine.1. Isolation of white blood cell (WBC) subsets. Experiments are proposed that require isolating specific subpopulations of WBC to look at their role in protecting indi-viduals from infection by the parasite T. Cruzi. Infection by this organism is a major cause of heart disease in South America. Other investigators will isolate subpoulations of WBC to look at their role in diseases such as Multiple Sclerosis. 2. Isolation of cells in distinct stages of the cell cycle. Experiments will be per-formed to test the effects of specific growth factors on regulating cell division. Regula-tion of cell growth is important both in development and in cancer, which is an uncon-trolled growth of tumor cells. A FACS machine is particularly well-suited to perform these kinds of experiments.3. Isolation of minority cell populations. In many cases, both within science and without, the rarest examples are often the most intriguing. Several investigators are in-terested in collecting rare, but important, cells from the body for analysis. Some of these cells help protect us against certain diseases, others may carry a disease. The FACS machine is very good at finding that one-in-a-thousand cell that is of interest.4. Enrichment of transfected cells. Transfected cells are cells that have been genetically altered in the laboratory so that they express new genes. One day it is hoped that people may be helped by giving them back such altered cells to compensate for a genetic abnormality. In the meantime the FACS machine is used in the lab to en-rich for these cells so that they can be analyzed in greater detailIn summary, a myriad of research and teaching projects at UCI will be facilitated and greatly enhanced by the acquisition of a state-of-the-art FACS machine. Unlike "all the king's horses and all the king's men" we believe we can put the pieces back together again, and in so doing gain a greater understanding of complex biological systems.

生物学中最强大的方法之一是还原论，将复杂系统分解成其组成部分的过程，以便更好地理解它们在整体上的相互作用。基于特定“标记”的表达，FACS机器允许从大的、不同的起始群体中富集特定的细胞亚群。这些表面标记物是通过结合荧光标记的特异性抗体来检测的，当暴露在FACS机器中的紫外激光下时，这些抗体会发光。UCI的研究人员也有不同的兴趣范围。这些兴趣可分为四大领域。这些仅仅是将受益于FACS机器的众多校园研究和教学项目中的一部分。白细胞亚群的分离。实验提出，需要分离特定的白细胞亚群，看看他们在保护个体免受T.克鲁兹寄生虫感染的作用。这种细菌的感染是南美心脏病的主要原因。其他研究人员将分离白细胞亚群，以观察它们在多发性硬化症等疾病中的作用。分离处于细胞周期不同阶段的细胞。实验将测试特定生长因子对细胞分裂的调节作用。细胞生长的调控在发育和癌症中都很重要，肿瘤细胞是一种不受控制的生长。FACS机器特别适合进行这类实验。少数细胞群体的分离。在许多情况下，无论是在科学领域还是在科学领域之外，最罕见的例子往往是最有趣的。一些研究人员对从人体中收集稀有但重要的细胞进行分析很感兴趣。这些细胞中的一些有助于保护我们免受某些疾病的侵害，另一些可能携带疾病。FACS机器非常善于发现千分之一的感兴趣的细胞。转染细胞的富集。转染细胞是指在实验室中经过基因改造，从而表达新基因的细胞。希望有一天，人们可以通过将这些改变的细胞交还给他们来补偿基因异常而得到帮助。同时，在实验室中使用FACS机器来丰富这些细胞，以便对它们进行更详细的分析。总之，UCI的无数研究和教学项目将通过获得最先进的FACS机器而得到促进和极大的加强。不像“所有国王的马和所有国王的人”，我们相信我们可以把碎片重新拼凑起来，这样做可以更好地理解复杂的生物系统。