Plant dicarboxylic acid homeostasis: on the specific physiological role of enzymes involved in malate decarboxylation

植物二羧酸稳态：苹果酸脱羧酶的特定生理作用

• 批准号: 138608285
• 负责人: Professorin Dr. Veronica Maurino
• 金额: --
• 依托单位: Abteilung Molekulare Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/138608285?language=en
• 关键词: Plant; dicarboxylic; acid; homeostasis; specific

Malate is an important intermediate in C3 metabolism as a component of the tricarboxylic acid and the glyoxylate cycles, it participates in stomatal movements, in the control of cytosolic pH, the maintenance of the cell electrical balance and is an important form of fixed carbon. Malate is present and undergoes transformations in all cell compartments due to the ubiquitous presence of enzymes involved in its synthesis or removal and of specific membrane carriers. This proposal, which is based on our recently published work, was designed to clarify several aspects of malate homeostasis in Arabidopsis thaliana with special focus on the elucidation of the biological function of malate decarboxylating enzymes (NAD-and NADP-ME). The planned work involves the generation and characterization of transgenic plants with altered malate and fumarate contents due to the lack or overexpression of NAD- and NADP-ME activities in specific cellular types or subcellular compartments. The effects of the overexpression or the lack of expression of a determined activity on the redirection of metabolic fluxes will be investigated by combining the measurement of metabolic fluxes with metabolic profiling, proteomic analysis and transcriptome analysis to assess the impact on C- and N-metabolism. The combined results of the different approaches are expected to shed new light on plant dicarboxylic acid metabolism.

苹果酸是C3代谢的重要中间体，作为三羧酸和乙醛酸循环的组分，它参与气孔运动，控制细胞溶质pH，维持细胞电平衡，并且是固定碳的重要形式。苹果酸存在于所有细胞隔室中，并由于参与其合成或去除的酶和特定膜载体的普遍存在而发生转化。这个建议，这是我们最近发表的工作的基础上，旨在澄清几个方面的苹果酸在拟南芥中的动态平衡，特别侧重于阐明苹果酸脱羧酶（NAD-和NADP-ME）的生物学功能。计划中的工作涉及的生成和表征的转基因植物与改变苹果酸和富马酸含量由于缺乏或过度表达的NAD-和NADP-ME活动在特定的细胞类型或亚细胞区室。将通过将代谢通量的测量与代谢谱、蛋白质组学分析和转录组学分析相结合来研究确定活性的过表达或缺乏表达对代谢通量重定向的影响，以评估对C-和N-代谢的影响。不同方法的综合结果预计将为植物二羧酸代谢提供新的线索。