A biotechnological approach to increase carbon assimilation in C3-plants
增加 C3 植物碳同化的生物技术方法
基本信息
- 批准号:25780487
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2006
- 资助国家:德国
- 起止时间:2005-12-31 至 2017-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Photorespiration starts with the competitive inhibition of CO2 fixation by O2 at the active site of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and results in a loss of at least 25% of the CO2 fixed in ambient air in C3-plants. Thus, photorespiration was considered a potentially wastefull proces that was limiting plant productivity. The prime function of the C2-pathway is to salvage glycolate 2-P by conversion to glycerate 3-P, which re-enters the C3-reductive cycle. We attempt to introduce two alternative and complete glycolate catabolic cycles into chloroplasts of the model plant Arabidopsis thaliana in order to release and re-fix the CO2 directly within the chloroplast, thereby reducing the oxygenase activity of Rubisco by increasing the CO2/O2 ratio. Thus, an autoregulatory cycle would be created which results in an attenuation of the photorespiratory pathway and an expected improvement in the efficiency of CO2 assimilation and consequently, faster biomass production. Analyses of transformants harboring fully operational glycolate cycles will also generate data to complement our understanding of function and regulation of the photorespiratory pathway and its interaction with other processes.
光呼吸开始于O2在核酮糖1,5-二磷酸羧化酶/加氧酶(Rubisco)活性位点对CO2固定的竞争性抑制,并导致C3植物中环境空气中固定的CO2损失至少25%。因此,光呼吸被认为是一种潜在的浪费过程,限制了植物的生产力。C2-途径的主要功能是通过转化为甘油酸3-P来挽救乙醇酸2-P,甘油酸3-P重新进入C3-还原循环。我们试图引入两个替代的和完整的乙醇酸分解代谢循环到模式植物拟南芥的叶绿体中,以释放和重新固定的CO2直接在叶绿体内,从而降低Rubisco的加氧酶活性,通过增加CO2/O2的比例。因此,将产生自动调节循环,其导致光呼吸途径的衰减和CO2同化效率的预期改善,从而更快地产生生物质。对具有完全可操作的乙醇酸循环的转化体的分析也将产生数据,以补充我们对光呼吸途径的功能和调节及其与其他过程的相互作用的理解。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Professorin Dr. Veronica Maurino其他文献
Professorin Dr. Veronica Maurino的其他文献
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{{ truncateString('Professorin Dr. Veronica Maurino', 18)}}的其他基金
Decoding the dual function of NAD-malic enzyme: from a universal role in malate respiration to a specific function in C4 photosynthesis
解读 NAD-苹果酸酶的双重功能:从苹果酸呼吸中的普遍作用到 C4 光合作用中的特定功能
- 批准号:
392217267 - 财政年份:2018
- 资助金额:
-- - 项目类别:
Research Grants
Deciphering the role of H2O2-signalling originating from different cellular compartments and cell types
解读源自不同细胞区室和细胞类型的 H2O2 信号传导的作用
- 批准号:
198630041 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Research Grants
Effects of chloroplastic originated-H2O2 in signalling and biotic interactions
叶绿体来源的 H2O2 对信号传导和生物相互作用的影响
- 批准号:
182723601 - 财政年份:2010
- 资助金额:
-- - 项目类别:
Heisenberg Fellowships
The impact of short-cutting photorespiration on carbon and nitrogen metabolism
短程光呼吸对碳氮代谢的影响
- 批准号:
134777978 - 财政年份:2009
- 资助金额:
-- - 项目类别:
Research Units
Plant dicarboxylic acid homeostasis: on the specific physiological role of enzymes involved in malate decarboxylation
植物二羧酸稳态:苹果酸脱羧酶的特定生理作用
- 批准号:
138608285 - 财政年份:2009
- 资助金额:
-- - 项目类别:
Research Grants
A genetic engineering approach to improve the carbon fixation in C3 plants by reducing the flux through the photorespiratory pathway. Consequences of the expression of novel activities in chloroplasts of A. thaliana
一种通过减少光呼吸途径通量来改善 C3 植物碳固定的基因工程方法。
- 批准号:
41426985 - 财政年份:2007
- 资助金额:
-- - 项目类别:
Research Grants
Re-cycling of glycolate in C3-chloroplasts: Biochemical and physiological effects on plant metabolism
C3-叶绿体中乙醇酸的再循环:对植物代谢的生化和生理影响
- 批准号:
5387331 - 财政年份:2002
- 资助金额:
-- - 项目类别:
Research Grants
Engineering of the photorespiratory carbon-cycle in C3-plants
C3 植物光呼吸碳循环工程
- 批准号:
5300602 - 财政年份:2001
- 资助金额:
-- - 项目类别:
Research Fellowships
Evolutionary optimization of enzymes for their operation in the C4 photosynthetic pathway: the case of NADP-malic enzyme
C4 光合作用途径酶的进化优化:以 NADP-苹果酸酶为例
- 批准号:
441941117 - 财政年份:
- 资助金额:
-- - 项目类别:
Research Grants
Divergent solutions to convergent evolution: elucidating the molecular strategies for co-option of the NAD-malic enzyme for C4 biochemistry
趋同进化的不同解决方案:阐明 C4 生物化学中 NAD-苹果酸酶共同选择的分子策略
- 批准号:
528182088 - 财政年份:
- 资助金额:
-- - 项目类别:
Research Grants
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