Deciphering the role of H2O2-signalling originating from different cellular compartments and cell types

解读源自不同细胞区室和细胞类型的 H2O2 信号传导的作用

• 批准号: 198630041
• 负责人: Professorin Dr. Veronica Maurino
• 金额: --
• 依托单位: Abteilung Molekulare Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/198630041?language=en
• 关键词: Deciphering; role; H2O2; signalling; originating

The genome-wide transcriptome analysis conducted in project MA2379/11-1 indicated that H2O2 produced in different cellular compartments modulates specific signalling pathways. First, we identified early (after 30 min of induction) responding genes to chloroplastic produced-H2O2 by qRT-PCR using ROS-responsive genes and transcription factor (TF) platforms (Balazadeh et al., 2012). Second, our wide transcriptomic analysis conducted after 8 h of induction of H2O2 production, allowed us to identify induced genes exclusively modulated by chloroplastic or peroxisomal produced H2O2 (Sewelan et al., 2014). Analysis of the data suggests that chloroplastic produced H2O2 has crucial sensory and signalling functions, while peroxisomal produced H2O2 most likely induces stress tolerance responses. During the next period, we will focus on selected players of chloroplastic H2O2-signalling networks that were identified in the previous work. A combination of genetic, molecular, and biochemical approaches will enable us to functionally characterize initially identified genes/genetic networks.On the one hand, we will specifically focus on the most highly up-regulated gene at 30 min after induction of H2O2 production. This gene encodes a putative U-box-type E3 ubiquitin ligase, the molecular function and biological role of which is still unknown. We will focus on the molecular and functional characterization of this protein to shed light on a novel link between ubiquitination and organellar-H2O2 signalling.On the other hand, our previous results indicate that while only three TF encoding-genes were specifically induced by H2O2 production in peroxisomes, 30 genes (14%) of the genes induced by chloroplastic produced H2O2 encoded TFs. The relatively large number of TF-encoding genes induced in GO5 plants indicates that H2O2 originating from chloroplasts has a critical specific signalling role controlling gene transcription. As these TFs are primary candidates to govern the H2O2-responsiveness of the other genes whose expression was up-regulated in the GO5 plants, we will focus on the role of these TFs during plant oxidative stress responses. A main aim of this subproject is the identification of target genes regulated by moderate levels of H2O2 produced from chloroplasts.

MA2379/11-1 项目中进行的全基因组转录组分析表明，不同细胞区室中产生的 H2O2 调节特定的信号传导途径。首先，我们使用 ROS 响应基因和转录因子 (TF) 平台通过 qRT-PCR 鉴定了对叶绿体产生的 H2O2 的早期（诱导 30 分钟后）响应基因（Balazadeh 等，2012）。其次，我们在诱导 H2O2 产生 8 小时后进行了广泛的转录组分析，使我们能够鉴定出仅受叶绿体或过氧化物酶体产生的 H2O2 调节的诱导基因（Sewelan 等，2014）。数据分析表明，叶绿体产生的 H2O2 具有重要的感觉和信号功能，而过氧化物酶体产生的 H2O2 最有可能诱导应激耐受反应。在接下来的一段时间内，我们将重点关注之前工作中确定的叶绿体 H2O2 信号网络的选定参与者。遗传、分子和生化方法的结合将使我们能够对最初识别的基因/遗传网络进行功能表征。一方面，我们将特别关注诱导 H2O2 产生后 30 分钟上调最高的基因。该基因编码一种假定的U-box型E3泛素连接酶，其分子功能和生物学作用仍不清楚。我们将重点关注该蛋白质的分子和功能表征，以揭示泛素化和细胞器 H2O2 信号传导之间的新联系。 另一方面，我们之前的结果表明，虽然只有 3 个 TF 编码基因是由过氧化物酶体中 H2O2 产生特异性诱导的，但叶绿体诱导的基因中有 30 个基因 (14%) 产生了 H2O2 编码的 TF。 GO5植物中诱导的相对大量的TF编码基因表明源自叶绿体的H2O2在控制基因转录方面具有关键的特定信号传导作用。由于这些 TF 是控制 GO5 植物中表达上调的其他基因的 H2O2 响应性的主要候选者，因此我们将重点关注这些 TF 在植物氧化应激反应中的作用。该子项目的主要目的是鉴定由叶绿体产生的中等水平的 H2O2 调节的靶基因。

项目成果

Activation of cyclic electron flow by hydrogen peroxide in vivo

• DOI: 10.1073/pnas.1418223112
• 发表时间: 2015-04
• 期刊: Proceedings of the National Academy of Sciences
• 作者: Deserah D. Strand;A. Livingston;Mio Satoh-Cruz;J. Froehlich;V. Maurino;D. Kramer

Biochemical control systems for small molecule damage in plants

• DOI: 10.1080/15592324.2018.1477906
• 发表时间: 2018-01-01
• 期刊: PLANT SIGNALING & BEHAVIOR
• 影响因子: 2.9
• 作者: Huedig, M.;Schmitz, J.;Maurino, V. G.
• 通讯作者: Maurino, V. G.