Deciphering the role of H2O2-signalling originating from different cellular compartments and cell types

解读源自不同细胞区室和细胞类型的 H2O2 信号传导的作用

• 批准号: 198630041
• 负责人: Professorin Dr. Veronica Maurino
• 金额: --
• 依托单位: Abteilung Molekulare Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/198630041?language=en
• 关键词: Deciphering; role; H2O2; signalling; originating

The genome-wide transcriptome analysis conducted in project MA2379/11-1 indicated that H2O2 produced in different cellular compartments modulates specific signalling pathways. First, we identified early (after 30 min of induction) responding genes to chloroplastic produced-H2O2 by qRT-PCR using ROS-responsive genes and transcription factor (TF) platforms (Balazadeh et al., 2012). Second, our wide transcriptomic analysis conducted after 8 h of induction of H2O2 production, allowed us to identify induced genes exclusively modulated by chloroplastic or peroxisomal produced H2O2 (Sewelan et al., 2014). Analysis of the data suggests that chloroplastic produced H2O2 has crucial sensory and signalling functions, while peroxisomal produced H2O2 most likely induces stress tolerance responses. During the next period, we will focus on selected players of chloroplastic H2O2-signalling networks that were identified in the previous work. A combination of genetic, molecular, and biochemical approaches will enable us to functionally characterize initially identified genes/genetic networks.On the one hand, we will specifically focus on the most highly up-regulated gene at 30 min after induction of H2O2 production. This gene encodes a putative U-box-type E3 ubiquitin ligase, the molecular function and biological role of which is still unknown. We will focus on the molecular and functional characterization of this protein to shed light on a novel link between ubiquitination and organellar-H2O2 signalling.On the other hand, our previous results indicate that while only three TF encoding-genes were specifically induced by H2O2 production in peroxisomes, 30 genes (14%) of the genes induced by chloroplastic produced H2O2 encoded TFs. The relatively large number of TF-encoding genes induced in GO5 plants indicates that H2O2 originating from chloroplasts has a critical specific signalling role controlling gene transcription. As these TFs are primary candidates to govern the H2O2-responsiveness of the other genes whose expression was up-regulated in the GO5 plants, we will focus on the role of these TFs during plant oxidative stress responses. A main aim of this subproject is the identification of target genes regulated by moderate levels of H2O2 produced from chloroplasts.

在MA2379/11-1项目中进行的全基因组转录组分析表明，不同细胞间产生的过氧化氢调节特定的信号通路。首先，我们使用ROS反应基因和转录因子(Tf)平台，通过qRT-PCR鉴定了早期(诱导30分钟后)对氯化塑料产生的过氧化氢做出反应的基因(Balazadeh等人，2012年)。其次，我们在诱导过氧化氢产生8小时后进行的广泛转录分析，使我们能够识别出仅受叶绿体或过氧化体产生的过氧化氢调节的诱导基因(Sewelan等人，2014年)。对数据的分析表明，氯塑料产生的过氧化氢具有关键的感觉和信号功能，而过氧化物体产生的过氧化氢最有可能诱导胁迫耐受反应。在接下来的一段时间里，我们将重点关注先前工作中发现的氯塑料过氧化氢信号网络的选定参与者。遗传、分子和生物化学方法的结合将使我们能够从功能上表征最初确定的基因/遗传网络。一方面，我们将专门关注诱导过氧化氢产生后30分钟上调最高的基因。该基因编码一种U-box型E3泛素连接酶，其分子功能和生物学作用尚不清楚。我们将集中于该蛋白的分子和功能特征，以阐明泛素化和细胞器-H_2O_2信号之间的新联系。另一方面，我们先前的结果表明，虽然只有3个TF编码基因被过氧化物体中的H_2O_2产生特异性地诱导，但在氯塑料诱导的基因中有30个基因(14%)产生H_2O_2编码T_f。在GO5植物中诱导的相对大量的TF编码基因表明，来自叶绿体的过氧化氢具有关键的特异信号作用，控制基因转录。由于这些转录因子是控制GO5植物中表达上调的其他基因对H_2O_2响应的主要候选者，我们将重点关注这些转录因子在植物氧化胁迫反应中的作用。这个子项目的一个主要目标是识别由叶绿体产生的中等水平的过氧化氢调节的目标基因。

项目成果

Activation of cyclic electron flow by hydrogen peroxide in vivo

• DOI: 10.1073/pnas.1418223112
• 发表时间: 2015-04
• 期刊: Proceedings of the National Academy of Sciences
• 作者: Deserah D. Strand;A. Livingston;Mio Satoh-Cruz;J. Froehlich;V. Maurino;D. Kramer

Biochemical control systems for small molecule damage in plants

• DOI: 10.1080/15592324.2018.1477906
• 发表时间: 2018-01-01
• 期刊: PLANT SIGNALING & BEHAVIOR
• 影响因子: 2.9
• 作者: Huedig, M.;Schmitz, J.;Maurino, V. G.
• 通讯作者: Maurino, V. G.