SGER: DNA-BASED NANOSTRUCTURE SELF-ASSEMBLY AND PATTERN REPLICATION

摘要CTS-0000539A。马宗达尔，美国加利福尼亚州 - 伯克利将研究用于复制纳米颗粒图案的基于 DNA 的新自组装方法的概念验证。 初始图案是使用扫描探针显微镜或电子束光刻创建的。 该图案被引入序列为 J'K' 的硫醇化单链 D​​NA 溶液中，以在基底上创建 J'K' 编码图案，并通过附着在基底上的 JK 编码纳米粒子来复制该图案。 溶液中引入的 DNA 连接酶仅共价结合纳米颗粒图案的 JK 部分。 通过将溶液加热至 70-80 摄氏度，共价键得以保留，而弱氢键被破坏，纳米粒子图案从基底图案上分离。 通过降低溶液温度然后再次加热，可以创建新的复制品。 所提出的方法允许对初始图案进行模型复制。该贡献是新颖的，可能对纳米结构系统的复制、自我复制和制造方法产生重要的长期影响。 该方法适用于 1-20 nm 范围，在该范围内，其他受控制造方法受到限制且尚不实用。

AbstractCTS-0000539A. Majumdar, U. California - BerkeleyThe proof of concept of a new DNA based self-assembly approach for replication of nanoparticle patterns will be investigated. The initial pattern is created using scanning probe microscope or electron-beam lithography. That pattern is introduced in a solution of thiolated single-stranded DNA of sequence J'K' to create a J'K' coded pattern on a substrate, which is replicated with a JK coded nanoparticles attached on the substrate. An enzyme DNA ligase introduced in the solution covalently binds only the sections JK of the nanoparticle pattern. By heating the solution to 70-80 degrees Celsius the covalent bonds remain while the weak hydrogen bonds are broken, and the nanoparticle pattern detaches from the substrate pattern. By decreasing the solution temperature and then heating again, new replicas are created. The proposed approach allows for model replication of the initial pattern.The contribution is novel and may have important long-term effects on replication, self-replication, and fabrication methods of nanostructured systems. The approach is applicable to the 1-20 nm range where other methods of controlled fabrication are limited and not yet practical.