SGER: DNA-BASED NANOSTRUCTURE SELF-ASSEMBLY AND PATTERN REPLICATION

SGER：基于 DNA 的纳米结构自组装和图案复制

• 批准号: 0000539
• 负责人: Arunava Majumdar
• 金额: $ 9.93万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0000539&HistoricalAwards=false
• 关键词: SGER; DNA; BASED; NANOSTRUCTURE; SELF

AbstractCTS-0000539A. Majumdar, U. California - BerkeleyThe proof of concept of a new DNA based self-assembly approach for replication of nanoparticle patterns will be investigated. The initial pattern is created using scanning probe microscope or electron-beam lithography. That pattern is introduced in a solution of thiolated single-stranded DNA of sequence J'K' to create a J'K' coded pattern on a substrate, which is replicated with a JK coded nanoparticles attached on the substrate. An enzyme DNA ligase introduced in the solution covalently binds only the sections JK of the nanoparticle pattern. By heating the solution to 70-80 degrees Celsius the covalent bonds remain while the weak hydrogen bonds are broken, and the nanoparticle pattern detaches from the substrate pattern. By decreasing the solution temperature and then heating again, new replicas are created. The proposed approach allows for model replication of the initial pattern.The contribution is novel and may have important long-term effects on replication, self-replication, and fabrication methods of nanostructured systems. The approach is applicable to the 1-20 nm range where other methods of controlled fabrication are limited and not yet practical.

摘要CTS-0000539 A。马宗达加州伯克利一个新的DNA为基础的自组装方法复制纳米粒子模式的概念证明将进行调查。 最初的图案是使用扫描探针显微镜或电子束光刻创建的。 将该图案引入序列J“K”的硫醇化单链DNA的溶液中，以在基底上产生J“K”编码图案，该图案用附着在基底上的JK编码纳米颗粒复制。 引入溶液中的酶DNA连接酶仅共价结合纳米颗粒图案的区段JK。 通过将溶液加热到70-80摄氏度，共价键保留，而弱氢键被破坏，并且纳米颗粒图案从衬底图案分离。 通过降低溶液温度，然后再次加热，创建新的副本。 所提出的方法允许初始图案的模型复制。该贡献是新颖的，并且可能对纳米结构系统的复制、自复制和制造方法产生重要的长期影响。 该方法适用于1-20 nm范围，其中其他受控制造方法受到限制并且尚未实用。