Quantitative Data Analysis and Graphics Facility: Storm 860 and Alpha Imagers

定量数据分析和图形设备：Storm 860 和 Alpha 成像仪

• 批准号: 0070321
• 负责人: James Forney
• 金额: $ 7.93万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2002-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0070321&HistoricalAwards=false
• 关键词: Quantitative; Data; Analysis; Graphics; Facility

0070321James D. ForneyPurdue Research FoundationQuantitative Data Analysis and Graphics Facility: Storm 860 and Alpha Imagers This award will fund the development of an interdepartmental facility for documentation and quantification of biochemical and molecular biological data. This facility will provide equipment and software to digitize data derived from most gel electrophoresis experiments and allows analysis of the resulting data. The procedures and techniques that can be analyzed will include: DNA and RNA sequencing and structure analysis, PCR-based assays including quantitative PCR and RFLP analysis, quantitative northern and Western blotting, Southern hybridizations, non-radioactive detection for research and teaching laboratories, colony counting and kinetic analyses of enzymatic reactions. The goal is to maximize use by making the facility as flexible and as accessible as possible.Equipment to be purchased includes two imagers capable of quantifying data imaged using autoradiography, fluorescent stains or labels, visible stains or labels, or simply using white lighting. These two components are the Storm 860 Imager (Molecular Dynamics) and the AlphaImager (AlphaInnotech). Sufficient computational equipment will be purchased to perform image and data analysis. The facility will be installed on the first floor of the centrally located Biochemistry building, which will provide easy access to researchers in several departments. The facility will be used for undergraduate and graduate level training in quantifying and analyzing data collected from biological assays.

00703211普渡大学研究基金会定量数据分析和图形设备：Storm 860和Alpha成像仪该合同将资助一个跨部门设备的开发，用于记录和定量生化和分子生物学数据。该设施将提供设备和软件来数字化大多数凝胶电泳实验的数据，并允许对结果数据进行分析。可以分析的程序和技术将包括：DNA和RNA测序和结构分析，基于PCR的分析，包括定量PCR和RFLP分析，定量northern和Western blotting， Southern杂交，研究和教学实验室的非放射性检测，菌落计数和酶促反应的动力学分析。我们的目标是通过使设施尽可能灵活和方便来最大限度地利用。需要购买的设备包括两个成像仪，能够使用放射自显影、荧光染色剂或标签、可见染色剂或标签或仅使用白色照明来定量成像数据。这两个组件是Storm 860成像仪（分子动力学）和AlphaImager （AlphaInnotech）。将购买足够的计算设备来进行图像和数据分析。该设施将安装在生物化学大楼的一楼，这将为几个部门的研究人员提供方便的访问。该设施将用于本科生和研究生水平的培训，用于量化和分析从生物分析中收集的数据。