Dissertation Research: Population Genetics of a Plant Disease Resistance Gene: Cf-2 in Natural Populations of L. pimpinellifolium

论文研究：植物抗病基因的群体遗传学：L. pimpinellifolium 自然群体中的 Cf-2

• 批准号: 0073082
• 负责人: Barbara Schaal
• 金额: $ 1万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-15 至 2003-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0073082&HistoricalAwards=false
• 关键词: Dissertation; Research; Population; Genetics; Plant

0073082Schaal & CaicedoPlant disease resistance genes, involved in the recognition of invading pathogens, have been a subject of intense research in the past decade. This has culminated in the identification of a handful of resistance genes, mostly in crop plants. However, little is known about the evolution of resistance genes in natural populations. This project will focus on the evolution of Cf-2, one of the few resistance genes to have been identified in a noncrop species. Allelic diversity of the Cf-2 gene in wild populations of Lycopersicon pimpinellifolium, a close relative of the tomato, will be surveyed to establish levels of within- and between-population diversity. This genetic variation will be analyzed with respect to the geneological relationships among Cf-2 alleles, geographical location, resistance phenotype, and the effects of natural selection. These analyses will reveal the microevolutionary processes that have shaped Cf-2's evolution.Plant resistance genes offer a unique opportunity to study how selection and other population-level processes affect an ecologically important trait at the molecular level. The study of adaptation at this level has traditionally been hindered by lack of knowledge of specific genes and their functions. Resistance genes thus emerge as ideal candidates to elucidate the evolutionary dynamics of molecular adaptation. Furthermore, knowledge of the mechanisms underlying resistance gene function and evolution is important for the continuing management of crop systems and the pests which threaten them.

植物抗病基因参与识别入侵的病原体，在过去的十年里一直是一个深入研究的课题。 这最终导致了少数抗性基因的鉴定，主要是在作物中。 然而，人们对自然群体中抗性基因的进化知之甚少。 该项目将重点关注Cf-2的进化，这是在非作物物种中发现的少数抗性基因之一。 将调查野生种群的Lycopersicon pimpinellifolium（番茄的近亲）的Cf-2基因的等位基因多样性，以确定种群内和种群间多样性的水平。 将从Cf-2等位基因、地理位置、抗性表型和自然选择的影响等方面分析这种遗传变异。 这些分析将揭示形成Cf-2进化的微进化过程。植物抗性基因提供了一个独特的机会来研究选择和其他群体水平的过程如何在分子水平上影响生态学上重要的性状。 传统上，由于缺乏对特定基因及其功能的了解，这一水平的适应性研究受到阻碍。 因此，抗性基因成为阐明分子适应的进化动力学的理想候选者。 此外，抗性基因功能和进化机制的知识对于作物系统和威胁它们的害虫的持续管理是重要的。