A Novel Calcium Sensor and Its Target Kinase in Arabidopsis

拟南芥中新型钙传感器及其靶激酶

基本信息

  • 批准号:
    0078233
  • 负责人:
  • 金额:
    $ 37.5万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2000
  • 资助国家:
    美国
  • 起止时间:
    2000-08-01 至 2003-07-31
  • 项目状态:
    已结题

项目摘要

A Novel Ca2+ Sensor and Its Target Kinase in Arabidopsis A number of stressful factors such as drought, cold, salinity, can severely hamper plant growth and development. To survive these extreme conditions, plants have evolved complex mechanisms to "monitor" the stress conditions and respond by changing their physiological and developmental programs. The long term goal of this project is to understand the signaling pathways that link the environmental stress factors to cellular responses in higher plants. Almost all extracellular signals, including plant hormones, light, stress factors, and pathogenic or symbiotic elicitors, can elicit Ca2+ pulses or transients which serve as "second messengers" in further signaling processes. Because different signals often induce distinct and specific cellular responses, an interesting question is how cells distinguish the Ca2+ messengers produced by different stimuli and respond accordingly. Studies suggest that signaling components that "sense" and "interpret" the Ca2+ parameters hold the key. The laboratory has identified a new family of Ca2+ sensor proteins that play a role in plant response to stress signals. One member of the family, AtCBL1, is strongly responsive to stress factors including drought, cold, and wounding. Another member, AtCBL4 or SOS3, is required for salt tolerance. To understand the molecular mechanism for AtCBL1 function, the laboratory has made a key finding that AtCBL1 specifically associates with a family of novel protein kinases that are found only in higher plants. These AtCBL1-interacting protein kinases (CIPKs) are highly related to each other and their kinase domains are most similar to SNF1 subfamily of protein kinases. However, all CIPKs contain a unique C-terminal non-kinase domain that is responsible for interaction with AtCBL1. Further studies with one of the CIPKs, CIPK1, show that AtCBL1 interaction with CIPK1 requires micromolar levels of Ca2+, suggesting that Ca2+-binding changes the conformation of AtCBL1 and triggers association with CIPK1. Kinase assays using recombinant CIPK1 determined that CIPK1 is a serine/threonine kinase but has unique cofactor preference. For instance, CIPK1 highly prefers Mn2+ for its activity and functions as a Mn2+-binding protein. This project will further explore the functional significance of AtCBL1-CIPK1 complex using a combination of biochemical, cell biology, and molecular genetic approaches. In the first objective, biochemical and cell biology approaches will be used to dissect the native form of CIPK1 holoenzyme and identify the functional substrates for CIPK1. In the second objective, molecular genetic approaches using transgenic plant and "knockout" mutant models will be used to unravel the function of AtCBL1-CIPK1 in plant growth and developmental processes especially under stress conditions. This study will establish a new paradigm for Ca2+ signal transduction, which will have major impact on cell biology in general and on signal transduction research in particular.
拟南芥中一种新型的钙离子感受器及其靶标蛋白激酶一系列胁迫因素,如干旱、寒冷、盐度等,都会严重阻碍植物的生长发育。为了在这些极端条件下生存下来,植物进化出了复杂的机制来“监控”胁迫条件,并通过改变它们的生理和发育程序来做出反应。这个项目的长期目标是了解高等植物中将环境胁迫因素与细胞反应联系起来的信号通路。几乎所有的细胞外信号,包括植物激素、光、胁迫因子和致病或共生激发子,都可以诱导钙脉冲或瞬时信号,在进一步的信号过程中充当“第二信使”。由于不同的信号通常会引起不同的和特定的细胞反应,一个有趣的问题是细胞如何区分不同刺激产生的钙信使并做出相应的反应。研究表明,“感觉”和“解释”钙离子参数的信号成分是关键。该实验室已经确定了一个新的钙离子感受器蛋白家族,它们在植物对胁迫信号的反应中发挥作用。该家族的一名成员AtCBL1对干旱、寒冷和伤害等应激因素反应强烈。另一个成员AtCBL4或SOS3是耐盐性所必需的。为了了解AtCBL1功能的分子机制,实验室取得了一个关键发现,即AtCBL1特定地与一个仅在高等植物中发现的新的蛋白激酶家族有关。这些AtCBL1相互作用的蛋白激酶(CIPK)相互之间高度相关,它们的激活域与SNF1亚家族的蛋白激酶最为相似。然而,所有的CIPK都含有一个独特的C末端非激酶域,负责与AtCBL1的相互作用。对其中一种CIPKs CIPK1的进一步研究表明,AtCBL1与CIPK1的相互作用需要微摩尔水平的钙离子,这表明钙结合改变了AtCBL1的构象并触发了与CIPK1的结合。用重组CIPK1进行的激酶分析表明,CIPK1是一种丝氨酸/苏氨酸激酶,但具有独特的辅因子偏好。例如,CIPK1高度偏好Mn2+的活性,并作为一种Mn2+结合蛋白发挥作用。该项目将结合生物化学、细胞生物学和分子遗传学方法,进一步探索AtCBL1-CIPK1复合体的功能意义。在第一个目标中,将使用生化和细胞生物学的方法来剖析CIPK1全酶的天然形式,并鉴定CIPK1的功能底物。在第二个目标中,我们将利用转基因植物和“基因敲除”突变模型的分子遗传学方法来揭示AtCBL1-CIPK1在植物生长发育过程中的功能,特别是在胁迫条件下。这项研究将建立一个新的钙信号转导范式,这将对细胞生物学特别是信号转导研究产生重大影响。

项目成果

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Sheng Luan其他文献

Isolation and characterization of a Raf gene from Chinese shrimp Fenneropenaeus chinensis in response to white spot syndrome virus infection
中国明对虾响应白斑综合症病毒感染的 Raf 基因的分离和表征
  • DOI:
    10.1016/j.fsi.2018.09.043
  • 发表时间:
    2018-12
  • 期刊:
  • 影响因子:
    4.7
  • 作者:
    Xupeng Li;Xianhong Meng;Sheng Luan;Kun Luo;Baoxiang Cao;Baolong Chen;Jie Kong
  • 通讯作者:
    Jie Kong
PHOTO-SENSITIVE LEAF ROLLING 1 encodes a polygalacturonase that modifies cell wall structure and drought tolerance in rice
光敏叶卷 1 编码一种多聚半乳糖醛酸酶,可改变水稻的细胞壁结构和耐旱性
  • DOI:
    10.1111/nph.16899
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    9.4
  • 作者:
    Guangheng Zhang;Xin Hou;Li Wang;Jing Xu;Jian Chen;Xue Fu;Nianwei Shen;Jinqiang Nian;Zhuanzhuan Jiang;Jiang Hu;Li Zhu;Yuchun Rao;Yafei Shi;Deyong Ren;Guojun Dong;Zhenyu Gao;Longbiao Guo;Qian Qian;Sheng Luan
  • 通讯作者:
    Sheng Luan
PHOTO-SENSITIVE LEAF ROLLING 1 encodes a polygalacturonase that modifies cell wall structure and drought tolerance in rice
  • DOI:
    DOI:10.1111/nph.16899
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
  • 作者:
    Guangheng Zhang;Xin Hou;Li Wang;Jing Xu;Jian Chen;Xue Fu;Nianwei Shen;Jinqiang Nian;Zhuanzhuan Jiang;Jiang Hu;Li Zhu;Yuchun Rao;Yafei Shi;Deyong Ren;Guojun Dong;Zhenyu Gao;Longbiao Guo;Qian Qian;Sheng Luan
  • 通讯作者:
    Sheng Luan
Erratum to: Vol. 35 No. 1 Publisher’s Erratum
  • DOI:
    10.1007/s00343-017-7466-6
  • 发表时间:
    2017-03-01
  • 期刊:
  • 影响因子:
    1.300
  • 作者:
    Shuangyan He;Mingxia He;Jürgen Fischer;Dongliang Yuan;Peng Xu;Tengfei Xu;Xianping Yang;Leonid Sokoletsky;Xiaodao Wei;Fang Shen;Juhong Zou;Maohua Guo;Songxue Cui;Wu Zhou;Dalu Gao;Guangzhen Jin;Xianqing Lü;Fuwen Qiu;Wendong Fang;Aijun Pan;Jing Cha;Shanwu Zhang;Jiang Huang;Tao Wang;Yongzhou Cheng;Xiaoyan Chen;Zhaopu Liu;Xiaohua Long;Zhishuai Hou;Haishen Wen;Jifang Li;Feng He;Qun Liu;Jinhuan Wang;Biao Guan;Qinglong Wang;Md. Shahjahan;Md. Farajul Kabir;Kizar Ahmed Sumon;Lipi Rani Bhowmik;Harunur Rashid;Shu Li;Kefu Yu;Jianxin Zhao;Yuexing Feng;Tianran Chen;Shun Zhou;Yichao Ren;Christopher M. Pearce;Shuanglin Dong;Xiangli Tian;Qinfeng Gao;Fang Wang;Liming Liu;Rongbin Du;Xiaoling Zhang;Shuanglin Dong;Shichun Sun;Song Feng;Jianing Lin;Song Sun;Fang Zhang;Zhipeng Zhang;Xuexi Tang;Haitian Tang;Jingjing Song;Jian Zhou;Hongjun Liu;Qixiang Wang;Kuimei Qian;Xia Liu;Yuwei Chen;Chengjun Sun;Fenghua Jiang;Wei Gao;Xiaoyun Li;Yanzhen Yu;Xiaofei Yin;Yong Wang;Haibing Ding;Zhongmin Sun;Yongqiang Wang;Pengcheng Yan;Hui Guo;Jianting Yao;Jiro Tanaka;Hiroshi Kawai;Na Song;Muyan Chen;Tianxiang Gao;Takashi Yanagimoto;Xia Lu;Sheng Luan;Jie Kong;Longyang Hu;Yong Mao;Shengping Zhong;Yan Liu;Weihong Zhao;Caiyan Li;Hui Miao
  • 通讯作者:
    Hui Miao
From receptor-like kinases to calcium spikes: what are the missing links?
从受体样激酶到钙尖峰:缺失的环节是什么?
  • DOI:
    10.1093/mp/ssu092
  • 发表时间:
    2014-10
  • 期刊:
  • 影响因子:
    27.5
  • 作者:
    Feng Yu;Wang Tian;Sheng Luan
  • 通讯作者:
    Sheng Luan

Sheng Luan的其他文献

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{{ truncateString('Sheng Luan', 18)}}的其他基金

Mechanisms of nutrient sensing and homeostasis in plants
植物营养感应和稳态机制
  • 批准号:
    2344945
  • 财政年份:
    2024
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Standard Grant
Regulation of nutrient homeostasis by the CBL-CIPK calcium sensor-kinase network in Arabidopsis
拟南芥中 CBL-CIPK 钙传感器激酶网络对营养稳态的调节
  • 批准号:
    2041585
  • 财政年份:
    2021
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Standard Grant
Conference: 2019 Organellar Channels and Transporters GRC/GRS; August 3-9, 2019; Mount Snow, VT
会议:2019细胞器通道和转运蛋白GRC/GRS;
  • 批准号:
    1906099
  • 财政年份:
    2019
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Standard Grant
Regulation of nutrient homeostasis by the CBL-CIPK calcium-based sensor-kinase network in plants
植物中 CBL-CIPK 钙基传感器激酶网络对营养稳态的调节
  • 批准号:
    1714795
  • 财政年份:
    2017
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Standard Grant
MCA-PGR: Genetic and Genomic Approaches to Understanding Low-K Tolerance in Rice
MCA-PGR:了解水稻低钾耐受性的遗传和基因组方法
  • 批准号:
    1339239
  • 财政年份:
    2013
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Continuing Grant
2010 Arabidopsis/AFGN Collaborative Project: An Exemplary Calcium Signaling Network in Plant Stress Responses
2010 拟南芥/AFGN 合作项目:植物应激反应中的钙信号网络示例
  • 批准号:
    0723931
  • 财政年份:
    2008
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Continuing Grant
Chloroplast Starch Metabolism: A New Regulatory Junction for Redox and Protein Phosphorylation
叶绿体淀粉代谢:氧化还原和蛋白质磷酸化的新调节接头
  • 批准号:
    0642220
  • 财政年份:
    2007
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Continuing Grant
Dissecting the CBL-CIPK Network in Plant Signal Transduction
剖析植物信号转导中的 CBL-CIPK 网络
  • 批准号:
    0316135
  • 财政年份:
    2003
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Continuing Grant
US-Germany Cooperative Research: Identification and Analysis of CBL1 Calcium Sensor/CIPK1 Target Proteins
美德合作研究:CBL1钙传感器/CIPK1靶蛋白的鉴定与分析
  • 批准号:
    0233146
  • 财政年份:
    2003
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Standard Grant
Collaborative Arabidopsis 2010 Project : Genomics and Proteomics Approaches to the Function of Tyrosine Phosphatases in Arabidopsis
拟南芥 2010 合作项目:拟南芥酪氨酸磷酸酶功能的基因组学和蛋白质组学方法
  • 批准号:
    0209823
  • 财政年份:
    2002
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Continuing Grant

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Calcium/NFAT/GLUT3通路调控糖酵解代谢在CAR-T细胞耗竭中的作用和机制研究
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Molecular mechanisms regulating the form and function of atypical calcium sensor proteins.
调节非典型钙传感器蛋白的形式和功能的分子机制。
  • 批准号:
    RGPIN-2020-07171
  • 财政年份:
    2022
  • 资助金额:
    $ 37.5万
  • 项目类别:
    Discovery Grants Program - Individual
Control of sensory habituation by an ultra-conserved calcium sensor
通过超保守的钙传感器控制感觉习惯
  • 批准号:
    BB/X00094X/1
  • 财政年份:
    2022
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The role of Neuronal Calcium Sensor-1/Frequenin2 in nociception
神经元钙传感器-1/Frequennin2 在伤害感受中的作用
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Molecular mechanisms regulating the form and function of atypical calcium sensor proteins.
调节非典型钙传感器蛋白的形式和功能的分子机制。
  • 批准号:
    RGPIN-2020-07171
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Regulation of nutrient homeostasis by the CBL-CIPK calcium sensor-kinase network in Arabidopsis
拟南芥中 CBL-CIPK 钙传感器激酶网络对营养稳态的调节
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    $ 37.5万
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Continuous calcium sensor patch for hypoparathyroid patients
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