Regulation of nutrient homeostasis by the CBL-CIPK calcium-based sensor-kinase network in plants

植物中 CBL-CIPK 钙基传感器激酶网络对营养稳态的调节

• 批准号: 1714795
• 负责人: Sheng Luan
• 金额: $ 90万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1714795&HistoricalAwards=false
• 关键词: Regulation; nutrient; homeostasis; CBL; CIPK

To grow, plants require a balanced supply of mineral nutrients, but today's soil is often deficient in essential minerals such as nitrogen, phosphorus, and potassium. To overcome this deficiency, crop production in the U.S. relies heavily on use of fertilizers, but this practice is costly, endangers environmental health and is not sustainable, because some essential minerals (e.g., potash and phosphorus rock) are limited natural resources. An alternative and more sustainable agricultural strategy is to breed plants with new traits that allow them to grow in nutrient-imbalanced soils. To achieve this goal requires better understanding of the molecular networks that allow plants to respond and adapt to the constantly changing status of mineral nutrients in the soil. This project addresses this need by studying how plants maintain the proper balance of mineral nutrients when the soil contains too much or too little of specific minerals, thus establishing the knowledgebase for breeding crops with little need of fertilizers, thereby supporting sustainable agriculture. The project will have educational benefits by providing opportunities for undergraduate and graduate students to engage in research and by reaching out to high school students, including those from underrepresented groups, through workshops and other activities aimed at promoting interest in biology research. Plants constantly monitor nutrient status in the soil and maintain nutrient homeostasis by controlling ion transport across the plasma membrane (for uptake) and tonoplast (for storage). However, the signaling mechanism linking nutrient status in the soil and membrane transport in plants is largely unknown. The principal investigator's laboratory discovered the CBL-CIPK calcium signaling network that functions in a number of cellular pathways including nutrient sensing. In addition to the CBL-CIPK pathway that targets a voltage-gated potassium channel in response to low-potassium status, their recent studies further identified a complex CBL-CIPK network that regulates vacuolar transport of a number of mineral nutrients. The findings have thus connected the CBL-CIPK signaling mechanism to the regulation of transport activities at the plasma membrane and vacuolar membrane, the two most important sites for nutrient homeostasis in plant cells. The proposed research will test the major hypothesis that a large network of "CBL-CIPK-ion channel" interactions couples the environmental signals, e.g., changes in nutrient status, to the regulation of membrane transport and nutrient homeostasis in plant cells. A combination of approaches, including genetic, biochemical, and electrophysiological tools, will be used to address connections of the CBL-CIPK network to potassium and magnesium homeostasis with a focus on understanding the coordination between the plasma membrane and vacuolar pathways in the regulation of nutrient balance.

为了生长，植物需要平衡的矿物质营养供应，但今天的土壤往往缺乏必需的矿物质，如氮，磷和钾。为了克服这一缺陷，美国的作物生产严重依赖于化肥的使用，但这种做法成本高昂，危及环境健康，而且是不可持续的，因为一些必需的矿物质（例如，钾和磷矿石）是有限的自然资源。 另一种更可持续的农业战略是培育具有新特性的植物，使它们能够在营养不平衡的土壤中生长。为了实现这一目标，需要更好地了解分子网络，使植物能够响应和适应土壤中不断变化的矿物质营养状态。 该项目通过研究当土壤中含有过多或过少的特定矿物质时，植物如何保持矿物质营养的适当平衡来满足这一需求，从而建立培育几乎不需要肥料的作物的知识库，从而支持可持续农业。 该项目将产生教育效益，为本科生和研究生提供从事研究的机会，并通过讲习班和其他旨在提高对生物学研究兴趣的活动，接触高中生，包括来自代表性不足群体的高中生。 植物不断监测土壤中的养分状况，并通过控制离子穿过质膜（用于吸收）和液泡膜（用于储存）的运输来维持养分动态平衡。然而，土壤中的营养状态和植物膜运输的信号机制在很大程度上是未知的。主要研究者的实验室发现了CBL-CIPK钙信号网络，该网络在包括营养传感在内的许多细胞途径中发挥作用。除了针对低钾状态的电压门控钾通道的CBL-CIPK途径外，他们最近的研究进一步确定了一个复杂的CBL-CIPK网络，该网络调节许多矿物质营养素的液泡运输。因此，这些发现将CBL-CIPK信号传导机制与质膜和液泡膜的运输活动调节联系起来，这是植物细胞中营养稳态的两个最重要的位点。拟议的研究将测试主要假设，即“CBL-CIPK-离子通道”相互作用的大型网络耦合环境信号，例如，营养状态的变化，调节植物细胞的膜运输和营养平衡。包括遗传，生物化学和电生理工具在内的方法组合将用于解决CBL-CIPK网络与钾和镁稳态的连接，重点是了解质膜和液泡途径之间的协调，以调节营养平衡。

项目成果

Rhythms of magnesium

• DOI: 10.1038/s41477-020-0706-3
• 发表时间: 2020-06
• 期刊: Nature Plants
• 影响因子: 18
• 作者: R. Tang;S. Luan

A Defective Vacuolar Proton Pump Enhances Aluminum Tolerance by Reducing Vacuole Sequestration of Organic Acids

有缺陷的液泡质子泵通过减少有机酸的液泡隔离来增强铝耐受性

• DOI: 10.1104/pp.19.00626
• 发表时间: 2019
• 期刊: Plant Physiol
• 作者: Zhang F;Yan X;Han X;Tang R;Chu M;Yang Y;Yang YH;Zhao F;Fu A;Luan S;Lan W
• 通讯作者: Lan W

Inner Envelope CHLOROPLAST MANGANESE TRANSPORTER 1 Supports Manganese Homeostasis and Phototrophic Growth in Arabidopsis

内膜叶绿体锰转运蛋白 1 支持拟南芥的锰稳态和光养生长

• DOI: 10.1016/j.molp.2018.04.007
• 发表时间: 2018
• 期刊: Molecular Plant
• 影响因子: 27.5
• 作者: Zhang Bin;Zhang Chi;Liu Congge;Jing Yanping;Wang Yuan;Jin Ling;Yang Lei;Zhao Fugeng;Lan Wenzhi;Zhang Bin;Zhang Chi;Fu Aigen;Shi Jisen;Luan Sheng;Lan WZ;Luan S
• 通讯作者: Luan S

Two tonoplast proton pumps function in Arabidopsis embryo development

两个液泡膜质子泵在拟南芥胚胎发育中发挥作用

• DOI: 10.1111/nph.16231
• 发表时间: 2019-11-14
• 期刊: NEW PHYTOLOGIST
• 影响因子: 9.4
• 作者: Jiang, Yu-Tong;Tang, Ren-Jie;Lin, Wen-Hui
• 通讯作者: Lin, Wen-Hui

Calcineurin B-Like Proteins CBL4 and CBL10 Mediate Two Independent Salt Tolerance Pathways in Arabidopsis

• DOI: 10.3390/ijms20102421
• 发表时间: 2019-05
• 期刊: International Journal of Molecular Sciences
• 影响因子: 5.6
• 作者: Yang Yang-Yang;Chi Zhang;R. Tang;Hai-xia Xu;Wenzhi Lan;Fugeng Zhao;S. Luan