Collaborative Arabidopsis 2010 Project : Genomics and Proteomics Approaches to the Function of Tyrosine Phosphatases in Arabidopsis

拟南芥 2010 合作项目：拟南芥酪氨酸磷酸酶功能的基因组学和蛋白质组学方法

• 批准号: 0209823
• 负责人: Sheng Luan
• 金额: $ 129.38万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0209823&HistoricalAwards=false
• 关键词: Collaborative; Arabidopsis; 2010; Project; Genomics

Reversible protein phosphorylation is the most common mechanism for cellular regulation in eukaryotic systems. Studies have demonstrated that serine/threonine phosphorylation plays a key role in the regulation of plant physiology and development. However, tyrosine phosphorylation, despite its overwhelming importance in animals, was largely neglected in higher plants. Recently the first protein tyrosine phosphatase (PTP) has been characterized from higher plants. Furthermore, a diverse group of 21 genes encoding putative PTPs have been identified from the Arabidopsis genome. Genetic analyses of 4 PTPs have demonstrated that they serve critical functions in plant development, hormone signaling, and stress responses. These studies have broken new ground in the understanding of tyrosine phosphorylation in plants and opened up a new area of signal transduction research. In this project, a multifaceted approach is taken that combines most advanced technology in proteomics and genomics with existing procedures in biochemistry, molecular and cellular biology, and genetics to address the function of tyrosine phosphatases in Arabidopsis. The cDNAs for all PTPs will isolated, enzymatic properties of these putative PTPs will be characterized, their expression pattern and subcellular localization will be determined, and genetic models will be produced and characterized. To understand the molecular mechanism underlying PTP function, the in vivo substrates of PTPs will be identified using mass spectrometry. A useful database on phosphoproteome of Arabidopsis will be built and will be publicly accessible through PlantP website (http://plantsp.sdsc.edu/).The goal of this project is to understand the function and interplay of all tyrosine phosphatases in plant growth and developmental processes at both whole plant and molecular levels. Such information will contribute to the overall goal of 2010 program: to understand the function of all Arabidopsis genes. This project will also integrate educational activities into the research program by establishing a summer workshop for high school biology teachers.

可逆的蛋白质磷酸化是真核生物系统中最常见的细胞调节机制。研究表明丝氨酸/苏氨酸磷酸化在植物生理和发育调控中起着关键作用。然而，酪氨酸磷酸化，尽管其在动物中的压倒性的重要性，在高等植物中很大程度上被忽视。最近，第一个蛋白酪氨酸磷酸酶（PTP）已被确定为高等植物。此外，已经从拟南芥基因组中鉴定出编码推定PTP的21个基因的不同组。对4种PTPs的遗传分析表明，它们在植物发育、激素信号传导和应激反应中发挥着关键作用。这些研究为植物酪氨酸磷酸化的研究开辟了新的领域，也为信号转导研究开辟了新的领域。在这个项目中，采取了多方面的方法，结合蛋白质组学和基因组学的最先进的技术与现有的程序，在生物化学，分子和细胞生物学，遗传学，以解决拟南芥中的酪氨酸磷酸酶的功能。将分离所有PTP的cDNA，表征这些推定PTP的酶性质，确定其表达模式和亚细胞定位，并产生和表征遗传模型。为了理解PTP功能的分子机制，将使用质谱法鉴定PTP的体内底物。本项目将建立一个拟南芥磷酸化蛋白质组数据库，并通过PlantP网站（http：//plantsp.sdsc.edu/）向公众开放。这些信息将有助于2010年计划的总体目标：了解所有拟南芥基因的功能。 该项目还将通过为高中生物教师建立暑期讲习班，将教育活动纳入研究计划。