Dissecting the CBL-CIPK Network in Plant Signal Transduction

剖析植物信号转导中的 CBL-CIPK 网络

• 批准号: 0316135
• 负责人: Sheng Luan
• 金额: $ 68万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0316135&HistoricalAwards=false
• 关键词: Dissecting; CBL; CIPK; Network; Plant

Plants cannot move and therefore they need to deal with environmental changes on-site. All plants that survive to this day have learned many ways to adapt to environmental changes, especially stressful conditions that affect their growth and development. If one understands how plants adapt to environmental stress, such knowledge can be used to engineer plants with strongest tolerance to the stress conditions--supercrops--to benefit agriculture. This project aims to find out the molecular mechanisms underlying plant responses to environmental stress factors. Almost all extracellular signals, including plant hormones, light, stress factors, and pathogenic or symbiotic elicitors, can elicit Calcium (Ca2+) pulses or transients which serve as "second messengers" in further signaling processes. Because different signals often induce distinct and specific cellular responses, an interesting question is how cells distinguish the Ca2+ messengers produced by different stimuli and respond accordingly. Studies suggest that signaling components that "sense" and "interpret" the Ca2+ parameters hold the key. Recent studies identified a new family of Ca2+ sensor proteins (CBLs) that play a role in plant response to stress signals. This family of calcium sensors works by interacting with a family of protein kinases (CIPKs). This project will use a number of approaches including biochemical, cell biological, genetics, and genomics tools, to understand how this CBL-CIPK network transduces the signals from the outside to the inside of plant cells. This research will involve undergraduate students who will learn first-hand biological research skills in the lab. In addition, this project will be educating high school biology teachers who will participate in lab experimental work during the summer. Such outreach activity will provide a link between K-12 schools and university research labs and will bring current research topics to the K-12 classrooms.

植物不能移动，因此它们需要在现场应对环境变化。所有存活到今天的植物都学会了许多适应环境变化的方法，尤其是影响它们生长和发育的压力条件。如果人们了解植物是如何适应环境压力的，就可以利用这些知识来设计对逆境条件具有最强耐受性的植物——超级作物——从而造福农业。本项目旨在探索植物对环境胁迫响应的分子机制。几乎所有的细胞外信号，包括植物激素、光、胁迫因子和致病或共生激发子，都可以引发钙（Ca2+）脉冲或瞬态，它们在进一步的信号传导过程中充当“第二信使”。由于不同的信号通常会诱导不同的和特定的细胞反应，一个有趣的问题是细胞如何区分由不同刺激产生的Ca2+信使并做出相应的反应。研究表明，“感知”和“解释”Ca2+参数的信号成分是关键。最近的研究发现了一个新的Ca2+传感器蛋白家族（CBLs），在植物对胁迫信号的反应中发挥作用。这个钙传感器家族通过与蛋白激酶家族（CIPKs）相互作用而起作用。该项目将使用多种方法，包括生物化学、细胞生物学、遗传学和基因组学工具，来了解这种CBL-CIPK网络如何将信号从外部传递到植物细胞内部。这项研究将涉及本科生，他们将在实验室学习第一手的生物学研究技能。此外，该项目将教育高中生物教师，他们将在夏季参加实验室实验工作。这种外展活动将在K-12学校和大学研究实验室之间建立联系，并将当前的研究课题带到K-12教室。