Functionality of Dilute-Environment Bacteria

稀环境细菌的功能

基本信息

  • 批准号:
    0085628
  • 负责人:
  • 金额:
    $ 34.5万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2000
  • 资助国家:
    美国
  • 起止时间:
    2000-10-01 至 2004-09-30
  • 项目状态:
    已结题

项目摘要

The objective of this effort is to understand properties of oligobacteria that allow growth in extremely dilute solutions in order to facilitate examination for extraterrestrial life. While dominant in terms of biomass in aquatic systems, typical forms are rarely if ever cultivated. New techniques such as growth from single cells give improved examples of typical forms and the strategy outlined is to generate cultures that may taken as examples for detailed examination. Procedures are designed to optimize culturability by observing numerous cultures inoculated with statistically abundant single cells. The populations appearing are visualized by flow cytometry and the number of species are assayed by molecular techniques using novel statistical formulations to assess viability. Sensitivity to the initiation of growth is examined by culture media amendments. Nutrient responses are examined within the framework of novel Janusian kinetics that relate organism construction to nutrient sequestering ability. Key questions involve artifactual contamination and substrate accelerated death. Fresh water will therefore be used to eliminate some salt-associated problems during media preparation. Temperature and species-species interactions will be evaluated as well. The cultures will be characterized physiologically and genetically. One objective is to obtain a representative culture for gene sequencing as an aid to a detailed understanding of the functionality of oligobacteria.
这项工作的目的是了解寡细菌的特性,这些细菌可以在极稀的溶液中生长,以便于检查外星生命。虽然在水生系统的生物量方面占主导地位,但典型形式很少被种植。单细胞生长等新技术提供了典型形式的改进示例,概述的策略是生成可作为详细检查示例的培养物。程序旨在通过观察接种有统计上丰富的单细胞的大量培养物来优化可培养性。出现的种群通过流式细胞术可视化,并通过分子技术使用新颖的统计公式来分析物种数量以评估生存能力。通过培养基修正来检查对生长起始的敏感性。在新的 Janusian 动力学框架内检查养分反应,该动力学将生物体结构与养分封存能力联系起来。关键问题涉及人为污染和底物加速死亡。因此,淡水将用于消除培养基制备过程中一些与盐相关的问题。温度和物种间相互作用也将被评估。培养物将具有生理和遗传特征。一个目标是获得用于基因测序的代表性培养物,以帮助详细了解寡细菌的功能。

项目成果

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Don Button其他文献

Don Button的其他文献

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{{ truncateString('Don Button', 18)}}的其他基金

Activity and Characteristics of the Dissolved Organic Carbon-Dependent Picoplankton over the Annual Cycle in a Subarctic Lake
亚北极湖泊溶解有机碳依赖的超微型浮游生物年周期的活动和特征
  • 批准号:
    0243913
  • 财政年份:
    2003
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant
Activity of Individual Aquatic Bacteria by Flow Cytometry; Instrumentation and Protocols
通过流式细胞术检测单个水生细菌的活性;
  • 批准号:
    0096748
  • 财政年份:
    2001
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant
Dynamic Properties of Picoplankton Over the Annual Cycle in Near-Polar Lakes
近极地湖泊微型浮游生物年度循环的动态特性
  • 批准号:
    9907776
  • 财政年份:
    1999
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Continuing Grant
LEXEN: Characteristics of Bacteria Native to Extremely Dilute Environments
LEXEN:极度稀释环境中原生细菌的特征
  • 批准号:
    9714298
  • 财政年份:
    1997
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant
RUI: Instrumental Methods for the Analysis of Aquatic Bacteria by Flow Cytometry
RUI:流式细胞术分析水生细菌的仪器方法
  • 批准号:
    9318432
  • 财政年份:
    1994
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant
Distribution, Nutrition, Physiology and Phylogenetics of Oligobacteria
寡杆菌的分布、营养、生理学和系统发育学
  • 批准号:
    9316731
  • 财政年份:
    1994
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Continuing Grant
Sensitivity, Distribution, Characterization and Production of Ultramicrobacteria
超微细菌的敏感性、分布、表征和生产
  • 批准号:
    9019625
  • 财政年份:
    1991
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Continuing Grant
REU: Refinement of Instrumentation, Protocols, and Calibrations for the Analysis of Marine Bacteria by Flow Cytometry
REU:流式细胞术分析海洋细菌的仪器、协议和校准的改进
  • 批准号:
    8822155
  • 财政年份:
    1989
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant
Development of a Facility for Flow Cytometry
流式细胞术设施的开发
  • 批准号:
    8518397
  • 财政年份:
    1986
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Continuing Grant
Development of a Facility for Flow Cytometry
流式细胞术设施的开发
  • 批准号:
    8411061
  • 财政年份:
    1984
  • 资助金额:
    $ 34.5万
  • 项目类别:
    Standard Grant

相似国自然基金

基于稀氮砷化镓(Dilute nitride GaNAs)的近红外自旋放大纳米线激光器的研究
  • 批准号:
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