Inquiry -based Learning through Team Projects in a Cell Biology Lab Course

通过细胞生物学实验室课程中的团队项目进行探究式学习

• 批准号: 0088185
• 负责人: Alison Morrison-Shetlar
• 金额: $ 8.81万
• 依托单位: The University of Central Florida Board of Trustees
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0088185&HistoricalAwards=false
• 关键词: Inquiry; based; Learning; through; Team

Biological Sciences (61) Through this project, we are implementing a new undergraduate laboratory course in Molecular Cell Biology (MCB). The MCB Lab is an upper division elective providing an intensive, inquiry-based lab. The inquiry-based format enables students to develop an understanding of the research process as a means to investigate cell structure and function. The MCB Lab is available to students in the Biology, Molecular Biology and Microbiology, and Chemistry Departments at the University of Central Florida in Orlando. The lab course integrates technology into the classroom through the use of video microscopy and modern computer imaging systems as well as the use of modern biochemical and molecular techniques and instrumentation. The MCB Lab adapts the use of green fluorescent protein (GFP) constructs to the undergraduate laboratory setting. The course adapts elements of the NSF project DUE#9952672, "Developing research-based laboratories in cell biology using green fluorescent protein as a unifying theme" and of the research literature using GFP fusion protein markers. During the semester course, students investigate the molecular basis of eukaryotic cell compartmentalization and cytoskeletal dynamics using various GFP fusion proteins as specific markers. This enables students to observe cellular behaviors in vivo using fluorescence video microscopy. GFP fusion proteins can also be detected biochemically and this feature enables students to integrate their view of eukaryotic cell structure and function by correlating microscopic localization with biochemical behavior of the GFP fusion proteins. The outcome of this work is a significant improvement in student learning through inquiry based activities and increased student enthusiasm and thus retention in the life sciences. This project addresses a local need that reflects a national concern: preparing undergraduates in the SMET areas for careers in the sciences, including professional schools, graduate programs, or research positions in academic or industrial settings. The MCB Lab addresses a limitation in the current curriculum by providing a modern inquiry based lab experience to complement lecture curriculum, enriching the undergraduate laboratory curriculum, providing a substantive undergraduate research experience, and preparing students for continued research in faculty research programs.

生物科学（61）通过这个项目，我们正在实施一个新的本科生实验室课程的分子细胞生物学（MCB）。MCB实验室是一个高年级选修课，提供了一个密集的，基于探究的实验室。基于探究的形式使学生能够了解研究过程，作为研究细胞结构和功能的一种手段。MCB实验室可供奥兰多中央佛罗里达大学生物学、分子生物学和微生物学以及化学系的学生使用。实验室课程通过使用视频显微镜和现代计算机成像系统以及使用现代生物化学和分子技术和仪器将技术融入课堂。MCB实验室使绿色荧光蛋白（GFP）构建体的使用适应本科实验室环境。该课程采用了NSF项目DUE#9952672的元素，“使用绿色荧光蛋白作为统一主题开发细胞生物学研究实验室”和使用GFP融合蛋白标记的研究文献。在本学期的课程中，学生将研究真核细胞区室化的分子基础和使用各种GFP融合蛋白作为特异性标记的细胞骨架动力学。这使学生能够使用荧光视频显微镜观察体内细胞行为。GFP融合蛋白也可以被生化检测，这一功能使学生能够通过将显微定位与GFP融合蛋白的生化行为相关联来整合他们对真核细胞结构和功能的看法。这项工作的成果是通过基于探究的活动，提高学生的热情，从而保留在生命科学的学生学习的显着改善。该项目解决了当地的需要，反映了国家的关注：准备本科生在SMET领域的职业生涯中的科学，包括专业学校，研究生课程，或在学术或工业环境的研究职位。MCB实验室通过提供基于现代探究的实验室体验来补充讲座课程，丰富本科实验室课程，提供实质性的本科研究经验，并为学生在教师研究计划中的继续研究做好准备，从而解决了当前课程的局限性。