Ecotype variant analysis of RNA editing trans-factors in plant mitochondria

植物线粒体RNA编辑反式因子的生态型变异分析

• 批准号: 145389441
• 负责人: Privatdozent Dr. Mizuki Takenaka
• 金额: --
• 依托单位: Laboratory of Plant Molecular Biology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/145389441?language=en
• 关键词: Ecotype; variant; analysis; RNA; editing

RNA Editing in mitochondria and plastids of plants alters specific C nucleotides to U in mRNAs and tRNAs. In mitochondrial RNA editing, we have recently identified the first of the specific factors, a PPR-protein, which is involved in RNA editing at three sites. This nuclear gene was characterized by utilizing the natural variation in RNA editing between ecotypes in Arabidopsis thaliana. We have identified further such RNA editing variants which we now want to exploit by locating the responsible ecotype-specific mutations and the genes with genetic tools. Biochemical assays will be used to investigate RNA binding of these factors and to find other interacting proteins. The project aims to identify and characterize further specificity factors of RNA editing in order to better understand the RNA editing process.

植物线粒体和质体中的RNA编辑将mRNA和tRNA中的特定C核苷酸改变为U。在线粒体RNA编辑中，我们最近确定了第一个特定因子，一种PPR蛋白，它在三个位点参与RNA编辑。该核基因的特征在于利用拟南芥中生态型之间RNA编辑的自然变异。我们已经确定了进一步的RNA编辑变体，我们现在希望通过定位负责的生态类型特异性突变和基因的遗传工具来利用这些变体。生化分析将用于研究这些因子的RNA结合，并找到其他相互作用的蛋白质。该项目旨在识别和表征RNA编辑的进一步特异性因素，以便更好地了解RNA编辑过程。