Ecotype analysis for functional domain mapping of plant RNA editing proteins

植物 RNA 编辑蛋白功能域图谱的生态型分析

• 批准号: 236617702
• 负责人: Privatdozent Dr. Mizuki Takenaka
• 金额: --
• 依托单位: Laboratory of Plant Molecular Biology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/236617702?language=en
• 关键词: Ecotype; analysis; functional; domain; mapping

RNA Editing in mitochondria and plastids of plants alters certain C nucleotides to U in mRNAs and tRNAs. A number of specific and general protein factors have recently been identified. Specific factors are characterized by repeat elements of about 35 amino acids which presumably contact individual nucleotides, the function of the other domains is unknown. The more general, likewise essential factors exhibit a conserved central domain, their function is not known. This project proposes to analyse the functional contributions of the various domains in these RNA editing proteins by exploiting the natural variation between ecotypes in Arabidopsis thaliana. Ecotype specific differences with effect on the function of the respective protein will reveal crucial information on the importance of individual amino acid positions. These insights into the tolerated variations of the protein surfaces and landscapes will allow predictions of the function and structure of these proteins. These will be tested experimentally.

植物线粒体和质体中的RNA编辑将mRNA和tRNA中的某些C核苷酸改变为U。最近已经鉴定了许多特异性和一般性蛋白质因子。特异性因子的特征在于约35个氨基酸的重复元件，其可能与单个核苷酸接触，其他结构域的功能尚不清楚。更一般的，同样的必需因子表现出保守的中心结构域，它们的功能尚不清楚。该项目提出通过利用拟南芥中生态型之间的自然变异来分析这些RNA编辑蛋白中各个结构域的功能贡献。影响各自蛋白质功能的生态型特异性差异将揭示关于单个氨基酸位置的重要性的关键信息。这些对蛋白质表面和景观的耐受变化的见解将允许预测这些蛋白质的功能和结构。这些将通过实验进行测试。

项目成果

MEF10 is required for RNA editing at nad2-842 in mitochondria of Arabidopsis thaliana and interacts with MORF8

• DOI: 10.1007/s11103-012-0003-2
• 发表时间: 2013-03-01
• 期刊: PLANT MOLECULAR BIOLOGY
• 影响因子: 5.1
• 作者: Haertel, Barbara;Zehrmann, Anja;Takenaka, Mizuki
• 通讯作者: Takenaka, Mizuki