Identification of plant RNA Editing factors in EMS mutants

EMS突变体中植物RNA编辑因子的鉴定

• 批准号: 88943013
• 负责人: Privatdozent Dr. Mizuki Takenaka
• 金额: --
• 依托单位: Laboratory of Plant Molecular Biology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/88943013?language=en
• 关键词: Identification; plant; RNA; Editing; factors

RNA Editing in mitochondria and plastids of plants alters specific C nucleotides to U in mRNAs and tRNAs. In mitochondrial editing so far none of the specific or general factors, most likely proteins, have yet been identified. Two novel genetic approaches have been initiated to identify mutants in RNA editing in Arabidopsis thaliana which will allow to characterize the respective nuclear genes. The first approach utilizes the natural variation between ecotypes and aims to exploit the well charactized tools to follow ecotype specific genotypic differences. The second, parallel approach identifies artificial mutations in RNA editing by a newly developed screening procedure and will follow the affected nuclear genes with the genetic tools of Arabidopsis thaliana. The project aims to identify the specificity factors of RNA editing.

植物线粒体和质体中的RNA编辑改变了mRNAs和tRNAs中特定的C核苷酸为U。到目前为止，在线粒体编辑中，还没有确定任何特定或一般的因素，最有可能的是蛋白质。两种新的遗传方法已经被用来在拟南芥的RNA编辑中识别突变体，这将使我们能够表征各自的核基因。第一种方法利用生态型之间的自然变异，旨在开发具有良好特性的工具来跟踪生态型特定的基因类型差异。第二种平行方法通过一种新开发的筛选程序识别RNA编辑中的人工突变，并将利用拟南芥的遗传工具跟踪受影响的核基因。该项目旨在确定RNA编辑的特异性因素。