Identification of plant RNA Editing factors in EMS mutants

植物线粒体和质体中的RNA编辑将mrna和trna中的特定C核苷酸改变为U。到目前为止，在线粒体编辑中，还没有发现任何特定的或一般的因素，最有可能是蛋白质。两种新的遗传方法已经开始鉴定拟南芥中RNA编辑的突变体，这将允许表征各自的核基因。第一种方法利用生态型之间的自然变异，旨在利用良好的特征工具来跟踪生态型特定的基因型差异。第二种平行方法通过新开发的筛选程序识别RNA编辑中的人工突变，并将使用拟南芥的遗传工具跟踪受影响的核基因。该项目旨在确定RNA编辑的特异性因子。

RNA Editing in mitochondria and plastids of plants alters specific C nucleotides to U in mRNAs and tRNAs. In mitochondrial editing so far none of the specific or general factors, most likely proteins, have yet been identified. Two novel genetic approaches have been initiated to identify mutants in RNA editing in Arabidopsis thaliana which will allow to characterize the respective nuclear genes. The first approach utilizes the natural variation between ecotypes and aims to exploit the well charactized tools to follow ecotype specific genotypic differences. The second, parallel approach identifies artificial mutations in RNA editing by a newly developed screening procedure and will follow the affected nuclear genes with the genetic tools of Arabidopsis thaliana. The project aims to identify the specificity factors of RNA editing.