U.S.-Hungary Biophysical Studies of the Enzyme Thiocapsa Roseopersicina Hydrogenases

美国-匈牙利对硫代桃红氢化酶的生物物理研究

• 批准号: 0103019
• 负责人: Michael Maroney
• 金额: $ 4.6万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0103019&HistoricalAwards=false
• 关键词: U.S.; Hungary; Biophysical; Studies; Enzyme

This U.S.-Hungarian research project involves Michael Maroney of the University of Massachusetts, Amherst, and Csaba Bagyinka of the Institute of Biophysics at the Hungarian Biological Research Center, Szeged. They intend to examine the biological processes in hydrogenase reaction mechanisms, which catalyze the reversible two-electron redox chemistry of dihydrogen. The project features work with the enzyme Thiocapsa roseopersicina.Theory suggests a number of possibilities for structures of T. roseopersicina catalytic intermediates which the international team will test by spectroscopic and other physical probes. To do so the researchers plan to use time-resolved FTIR studies for determining the species involved in catalysis. They will characterize interconversions and their rates, hoping to detect kinetic intermediates and follow evolving thermodynamic microstates. The US-Hungarian partners specifically seek answers to questions about what roles protons play in determining the charge on active sites, what redox states are involved in catalysis, or whether protonated cysteines are present. If successful, findings may provide clues applicable to conversion of chemical energy to electricity for fuel cells and to storage of solar energy in chemical form via photochemical hydrogen production. This international project in biophysics research fulfills the program objective of advancing scientific knowledge by enabling experts in the Untied States and Central Europe to combine complementary talents and share research resources in areas of strong mutual interest and competence.

这个美国-匈牙利的研究项目涉及阿默斯特马萨诸塞州大学的迈克尔·马罗尼和塞格德匈牙利生物研究中心生物物理研究所的乔巴·巴金卡。他们打算研究氢化酶反应机制中的生物过程，该机制催化二氢的可逆双电子氧化还原化学。 该项目的特点是与酶Thiocapsa roseopersicina的工作。rosepersicina催化中间体，国际团队将通过光谱和其他物理探针进行测试。 为此，研究人员计划使用时间分辨FTIR研究来确定参与催化的物种。 他们将描述相互转化及其速率，希望检测动力学中间体并跟踪不断发展的热力学微观状态。 美国和匈牙利的合作伙伴特别寻求有关质子在确定活性位点上的电荷方面发挥什么作用，催化中涉及什么氧化还原状态，或者是否存在质子化半胱氨酸等问题的答案。 如果成功的话，这些发现可能会为燃料电池将化学能转化为电能以及通过光化学制氢以化学形式储存太阳能提供线索。这个生物物理研究的国际项目实现了促进科学知识的计划目标，使美国和中欧的专家能够联合收割机互补人才，并在共同感兴趣和能力强的领域共享研究资源。