SGER: Construction of a Whole Genome Micro-Array for the Marine Cyanobacterium Prochlorococcus

SGER：海洋蓝藻原绿球菌全基因组微阵列的构建

• 批准号: 0107472
• 负责人: Sallie Chisholm
• 金额: $ 9.62万
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0107472&HistoricalAwards=false
• 关键词: SGER; Construction; Whole; Genome; Micro

Prochlorococcus, a marine cyanobacterium, is a dominant phototroph in the world's oceans which contribute up to 50% of oceanic primary productivity. The complete genome sequences of two Prochlorococcus isolates have recently been completed by the Joint Genome Institute of the DOE. This study will develop complete genome microarrays to study gene expression in these isolates. This technology will not only help to identify the numerous unidentified genes in these organisms that have no homologues in sequence databases, but they will also provide insights into the environmental variables that have driven niche diversification in this group. Prochlorococcus thrives due to the existence of multiple ecotypes, each slightly specialized to thrive optimally under a slightly different set of environmental conditions. Pending the success of this project, a full-blown study will be conducted applying this array to the evolution and ecology of this organism. The array will also be made available to others.

海洋蓝细菌ProChorococcus是世界海洋中的主要光营养，占海洋初级生产力的50％。 最近，美国能源部联合基因组研究所完成了两个甲氯环球菌的完整基因组序列。 这项研究将开发完整的基因组微阵列，以研究这些分离株中的基因表达。 这项技术不仅将有助于确定这些生物体中没有序列数据库同源物的众多未识别基因，而且还将提供对驱动该组中利基多样化的环境变量的见解。由于存在多种生态型，因此欧洲氯环球菌蓬勃发展，每个生态型都在略有不同的环境条件下略有专门为最佳蓬勃发展。 在该项目的成功之前，将进行一项全面研究，将此阵列应用于该生物的进化和生态。 该阵列也将提供给其他人。