EDGE FGT: Genetic Tools for Picocyanobacteria that Dominate the Oceans

EDGE FGT:主宰海洋的微微蓝藻的遗传工具

基本信息

  • 批准号:
    2035181
  • 负责人:
  • 金额:
    $ 55万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-05-01 至 2024-04-30
  • 项目状态:
    已结题

项目摘要

As the most abundant photosynthetic organism on the planet, the marine cyanobacterium Prochlorococcus plays a central role in providing energy and nutrients to ocean food webs. This species alone fixes approximately as much carbon dioxide annually as global croplands. Understanding the link between the diverse genes of Prochlorococcus and its central role in ocean metabolism is hindered by our inability to modify its genes to test various hypotheses. Building on recent progress, this project aims to develop two distinct but parallel approaches to the challenge. In the first, barriers to the introduction of foreign DNA to Prochlorococcus will be bypassed by exploiting a system of newly discovered genetic elements that are found in some Prochlorococcus strains. In the second, approaches to trigger mechanisms that allow cells to take up and integrate new genes into their cellular machinery will be developed. Once developed, these approaches would allow better understanding of the link between Prochlorococcus genes and the ocean environment, which is critical for characterizing the role ocean processes play in regulating the global carbon cycle. These advances will also provide a blueprint for the development of similar methodologies for studying other microbial organisms. Finally, developing these technologies is a critical first step in enabling Prochlorococcus as a potential chassis for artificial photosynthesis. Since it has the smallest genome of any photosynthetic cell, Prochlorococcus is already the most basic photosynthetic machine occurring in nature, making it the ideal candidate for developing the tools that would enable bioengineering. The tools this project aims to develop would provide immense potential for new inquiry and biotechnology applications. This project provides support for graduate students, a postdoctoral research associate, and two technicians. Over the past three decades, the cyanobacterium Prochlorococcus, notable for its numerical dominance of the oceans and enormous genetic diversity, has been developed as a model organism for cross-scale systems biology. While each cell has around 2000 genes, the total unique gene content of the global Prochlorococcus ‘collective’ likely exceeds 80,000 genes, most of which have unknown function. Thus, despite their central role in ocean metabolism, the relationship between Prochlorococcus’ diverse genetic content, and the selective pressures that have shaped it, is only beginning to be understood. Progress on this challenge has been stalled by the lack of a genetic system. In previous work supported by the EDGE program, the researchers developed a method of introducing exogenous DNA into Prochlorococcus cells, which represented a significant step forward in genomic manipulation. In the process, a system of mobile genetic elements was discovered, which represent a promising new tool for integration of exogenous DNA into the Prochlorococcus, electroporation delivered tycheposons that are constructed with native genetic elements. A second approach to be developed utilizes a novel transposon-based screen to identify conditions for triggering natural competence activity among Prochlorococcus and will result in a genome-wide map of promising integration sites. Both approaches have the advantage of utilizing DNA from Prochlorococcus itself, which should more effectively bypass cell defenses against exogenous DNA.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
作为地球上最丰富的光合生物,海洋蓝藻原绿球藻在为海洋食物网提供能量和营养方面发挥着核心作用。仅这一物种每年固定的二氧化碳量就相当于全球农田。了解原绿球藻的不同基因之间的联系及其在海洋代谢中的核心作用是由于我们无法修改其基因来测试各种假设而受到阻碍。在最近取得的进展的基础上,该项目旨在制定两种不同但平行的方法来应对这一挑战。在第一个中,将通过利用在一些原绿球藻菌株中发现的新发现的遗传元件的系统来绕过将外源DNA引入原绿球藻的障碍。第二,将开发触发机制的方法,使细胞能够吸收新基因并将其整合到细胞机器中。一旦开发出来,这些方法将有助于更好地了解原绿球藻基因与海洋环境之间的联系,这对于描述海洋过程在调节全球碳循环方面的作用至关重要。这些进展还将为研究其他微生物的类似方法的发展提供蓝图。最后,开发这些技术是使原绿球藻成为人工光合作用潜在基础的关键的第一步。由于它具有任何光合细胞中最小的基因组,原绿球藻已经是自然界中最基本的光合机器,使其成为开发生物工程工具的理想候选者。该项目旨在开发的工具将为新的调查和生物技术应用提供巨大的潜力。该项目为研究生,博士后研究助理和两名技术人员提供支持。 在过去三十年中,以其在海洋中的数量优势和巨大的遗传多样性而闻名的蓝细菌原绿球藻已被开发为跨尺度系统生物学的模式生物。虽然每个细胞有大约2000个基因,但全球原绿球藻“集体”的独特基因含量可能超过80,000个基因,其中大部分基因的功能未知。因此,尽管原绿球藻在海洋新陈代谢中发挥着核心作用,但它们的多样遗传内容与塑造它的选择压力之间的关系才刚刚开始被理解。由于缺乏遗传系统,这一挑战的进展一直停滞不前。在EDGE计划支持的先前工作中,研究人员开发了一种将外源DNA引入原绿球藻细胞的方法,这代表了基因组操作的重要一步。在此过程中,发现了一个移动的遗传元件系统,它代表了一个有前途的新工具,外源DNA整合到原绿球藻,电穿孔传递的类型座子,构建与天然遗传元件。第二种方法将开发利用一种新的转座子为基础的屏幕,以确定触发原绿球藻之间的自然能力活动的条件,并会导致在全基因组范围内有前途的整合位点的地图。这两种方法都具有利用原绿球藻本身DNA的优势,这应该更有效地绕过细胞对外源DNA的防御。该奖项反映了NSF的法定使命,并且通过使用基金会的知识价值和更广泛的影响进行评估,被认为值得支持审查标准。

项目成果

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Sallie Chisholm其他文献

Sallie Chisholm的其他文献

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{{ truncateString('Sallie Chisholm', 18)}}的其他基金

Collaborative Research: EDGE-FGT: Furthering Progress on a Genetic System for the Oceans' Most Abundant Phototrophs
合作研究:EDGE-FGT:海洋最丰富的光养生物遗传系统的进一步进展
  • 批准号:
    2319332
  • 财政年份:
    2023
  • 资助金额:
    $ 55万
  • 项目类别:
    Standard Grant
IOS EDGE: Development of genetic tools for the dominant phototroph in the sea
IOS EDGE:开发海洋中优势光养生物的遗传工具
  • 批准号:
    1645061
  • 财政年份:
    2017
  • 资助金额:
    $ 55万
  • 项目类别:
    Standard Grant
Membrane vesicles produced by marine bacteria: origins, distributions, and functions
海洋细菌产生的膜囊泡:起源、分布和功能
  • 批准号:
    1356460
  • 财政年份:
    2014
  • 资助金额:
    $ 55万
  • 项目类别:
    Standard Grant
Microevolution and population dynamics of Prochlorococcus cells in the ocean: Insights through single-cell genomics
海洋中原绿球藻细胞的微进化和种群动态:通过单细胞基因组学的见解
  • 批准号:
    1145734
  • 财政年份:
    2012
  • 资助金额:
    $ 55万
  • 项目类别:
    Standard Grant
Nitrate Assimilation and the Ecology of Prochlorococcus: Features and Implications of Intraspecific Diversity in a Model Marine Phototroph
硝酸盐同化和原绿球藻生态学:模型海洋光养生物种内多样性的特征和意义
  • 批准号:
    1153588
  • 财政年份:
    2012
  • 资助金额:
    $ 55万
  • 项目类别:
    Standard Grant
The Ecology of Prochlorococcus: Toward a Model System for Microbial Oceanography
原绿球藻的生态学:建立微生物海洋学模型系统
  • 批准号:
    0425602
  • 财政年份:
    2004
  • 资助金额:
    $ 55万
  • 项目类别:
    Standard Grant
Collaborative Research: Southern Ocean Iron Experiment (SOFeX): Mesoscale Iron Fertilization Effects on Plankton Community Structure, Growth and Zooplankton Grazing
合作研究:南大洋铁实验(SOFeX):中尺度铁施肥对浮游生物群落结构、生长和浮游动物放牧的影响
  • 批准号:
    0000330
  • 财政年份:
    2001
  • 资助金额:
    $ 55万
  • 项目类别:
    Standard Grant
SGER: Construction of a Whole Genome Micro-Array for the Marine Cyanobacterium Prochlorococcus
SGER:海洋蓝藻原绿球菌全基因组微阵列的构建
  • 批准号:
    0107472
  • 财政年份:
    2001
  • 资助金额:
    $ 55万
  • 项目类别:
    Standard Grant
Regulation of Population Dynamics of Prochlorococcus and Synechococcus Ecotypes in Diverse Oceanoic Ecosystems
不同海洋生态系统中原绿球藻和聚球藻生态型种群动态的调控
  • 批准号:
    9820035
  • 财政年份:
    1999
  • 资助金额:
    $ 55万
  • 项目类别:
    Continuing Grant
Metal Speciation and Cyanolbacterial Ecology in the Sargasso Sea
马尾藻海的金属形态和蓝藻生态学
  • 批准号:
    9701681
  • 财政年份:
    1997
  • 资助金额:
    $ 55万
  • 项目类别:
    Continuing Grant

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Collaborative Research: EDGE FGT: Genome-wide knock-out mutant libraries for the microcrustacean Daphnia
合作研究:EDGE FGT:微甲壳动物水蚤的全基因组敲除突变体文库
  • 批准号:
    2324639
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  • 批准号:
    10673453
  • 财政年份:
    2023
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Collaborative Research: EDGE-FGT: Furthering Progress on a Genetic System for the Oceans' Most Abundant Phototrophs
合作研究:EDGE-FGT:海洋最丰富的光养生物遗传系统的进一步进展
  • 批准号:
    2319334
  • 财政年份:
    2023
  • 资助金额:
    $ 55万
  • 项目类别:
    Standard Grant
EDGE FGT: RNAi-based tools to unlock functional genomics of obligate oomycete plant pathogens
EDGE FGT:基于 RNAi 的工具,用于解锁专性卵菌植物病原体的功能基因组学
  • 批准号:
    2319757
  • 财政年份:
    2023
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    $ 55万
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Collaborative Research: EDGE FGT: Development of a Comprehensive Selection Library to Reconcile Core Metabolic Knowledge Gaps
合作研究:EDGE FGT:开发综合选择库以弥合核心代谢知识差距
  • 批准号:
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  • 财政年份:
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  • 资助金额:
    $ 55万
  • 项目类别:
    Standard Grant
Collaborative Research: EDGE FGT: Development of a Comprehensive Selection Library to Reconcile Core Metabolic Knowledge Gaps
合作研究:EDGE FGT:开发综合选择库以弥合核心代谢知识差距
  • 批准号:
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    Standard Grant
Collaborative Research: EDGE-FGT: Furthering Progress on a Genetic System for the Oceans' Most Abundant Phototrophs
合作研究:EDGE-FGT:海洋最丰富的光养生物遗传系统的进一步进展
  • 批准号:
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合作研究:EDGE FGT:促进多样化、新兴模型被子植物的转化和基因组资源
  • 批准号:
    2335775
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    $ 55万
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EDGE FGT: Genome-editing tools for keystone freshwater heterotrophs
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合作研究:EDGE FGT:Ipomoea(牵牛花)遗传和功能基因组学资源的开发
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