Regulation of osmolyte synthesis in the moderate halophile Halobacillus halophilus

中度嗜盐菌嗜盐杆菌渗透调节剂合成的调节

• 批准号: 150413131
• 负责人: Professor Dr. Volker Müller
• 金额: --
• 依托单位: Arbeitskreis Molekulare Mikrobiologie und Bioenergetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/150413131?language=en
• 关键词: Regulation; osmolyte; synthesis; moderate; halophile

The moderately halophilic, chloride-dependent bacterium Halobacillus halophilus copes with the salinity in its environment by the intracellular production of compatible solutes in molar concentrations. Interestingly, it switches its osmolyte strategy from glutamate/glutamine at moderate to proline at high salinities and we have obtained evidence for a two-step process involved in sensing salinity with chloride and glutamate as key players. Here, we will address the molecular basis of sensing and responding to changing salinities using subcellular systems. The glutamine synthetase activity in whole cells was characterized as a chloride and salt sensor and the responsible enzyme (GlnA2) was identified and purified. We will address the mode of sensing and activation by analyzing purified GlnA2 with biochemical, biophysical, molecular and genetic tools. The genes involved in salinity-dependent proline biosynthesis were also identified and their expression was shown to be stimulated to a great extent by glutamate. In the second part of the project we will focus on the mechanism of glutamate-activated gene expression. Furthermore, the regulation of production of proline biosynthetic proteins as well as their enzymatic activity will be studied.

中度嗜盐、氯化物依赖性细菌嗜盐杆菌通过在细胞内产生摩尔浓度的相容溶质来应对其环境中的盐度。有趣的是，它切换其渗透策略从谷氨酸/谷氨酰胺在中等到脯氨酸在高盐度，我们已经获得了证据的两个步骤的过程中参与感测盐度与氯化物和谷氨酸盐作为关键球员。在这里，我们将解决的分子基础上的传感和响应变化的盐度使用亚细胞系统。谷氨酰胺合成酶活性在整个细胞的特点是作为一个氯离子和盐的传感器和负责酶（GlnA 2）进行了鉴定和纯化。我们将通过生物化学、生物物理学、分子和遗传学工具分析纯化的GlnA 2来解决传感和激活模式。参与盐依赖性脯氨酸生物合成的基因也被确定，其表达在很大程度上被谷氨酸刺激。在本项目的第二部分中，我们将关注谷氨酸激活基因表达的机制。此外，还将研究脯氨酸生物合成蛋白质的产生及其酶活性的调节。

项目成果

Bioenergetics of the Moderately Halophilic Bacterium Halobacillus halophilus: Composition and Regulation of the Respiratory Chain

• DOI: 10.1128/aem.00855-13
• 发表时间: 2013-04
• 期刊: Applied and Environmental Microbiology
• 影响因子: 4.4
• 作者: N. Pade;Saskia K�cher;Markus Roe�ler;Inga H�nelt;Volker M�ller

Adjusting membrane lipids under salt stress: the case of the moderate halophilic organism Halobacillus halophilus.

• DOI: 10.1111/j.1462-2920.2012.02870.x
• 发表时间: 2013-04
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: P. Lopalco;R. Angelini;S. Lobasso;S. Köcher;Melanie Thompson;V. Müller;A. Corcelli

Chloride and organic osmolytes: a hybrid strategy to cope with elevated salinities by the moderately halophilic, chloride-dependent bacterium Halobacillus halophilus.

氯化物和有机渗透剂：中度嗜盐、氯化物依赖性细菌嗜盐杆菌应对盐度升高的混合策略

• DOI: 10.1111/j.1462-2920.2012.02770.x
• 发表时间: 2012
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Pfeiffer;Schuster;Müller;Oesterhelt
• 通讯作者: Oesterhelt

Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus

谷氨酰胺合成酶 2 对于嗜盐芽孢杆菌中相容性溶质的生物合成不是必需的

• DOI: 10.3389/fmicb.2014.00168
• 发表时间: 2014
• 期刊: Frontiers in Microbiology
• 影响因子: 5.2
• 作者: Shiyan;Thompson;Köcher;Tausendschön;Santos;Hänelt;Müller
• 通讯作者: Müller