Optical Imaging and Fluorescence Microscopy Enhancement

光学成像和荧光显微镜增强

• 批准号: 0115927
• 负责人: Ellen Brisch
• 金额: $ 11.62万
• 依托单位: Minnesota State University Moorhead
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0115927&HistoricalAwards=false
• 关键词: Optical; Imaging; Fluorescence; Microscopy; Enhancement

A grant has been awarded to Dr. Ellen Brisch at Minnesota State University Moorhead (MSUM) to build on our microscopy capabilities by upgrading our current Olympus IX-70 brightfield/phase contrast microscope. The funding provided by this grant will allow us to incorporate cell injection, image capture, image analysis and high quality figure preparation capabilities into our teaching and research. This equipment will be shared between the PI; Dr. Ellen Brisch, and three Co-PIs; Dr. Chris Chastain, Dr. Joseph Provost and Dr. Mark Wallert. The equipment and instrumentation this grant will fund includes: a cell injection system, additional immunofluorescent filter cubes and high quality 100x objectives, a software package which will enable us to detect and quantify subtle changes in pH, an isolation table and motorized stage, state of the art Macintosh computers for image analysis and figure preparation, and microscopy training workshops. All four investigators are engaged in undergraduate teaching and training. Our current goals are to provide a state of the art education to our undergraduates by training them on state of the art equipment and also to bring our research programs to a higher level. The research programs led by the investigators vary. Dr. Brisch's research program focuses on how the replication and segregation of a critical cellular organelle, the mitochondrion, is coordinated during cell division. Dr. Brisch plans to examine a collection of yeast cell cycle mutants--yeast cells that fail to complete the cell division cycle correctly--by staining mitochondria and observing them with fluorescence microscopy. Dr. Chastain's research program focuses on understanding enzyme activity in the C4 photosynthetic pathway. Dr. Chastain plans to examine the distribution of a C4 photosynthetic enzyme in a C3 plant by employing immunolocalization studies. Drs. Wallert and Provost collaborate significantly in their research efforts. Their research program focuses on how mitogenic signaling pathways alter intracellular pH. They plan to perform cell injection experiments by microinjecting a variety of interfering agents designed to alter specific cell signaling pathways. They plan to quantitate the presence or absence of subtle pH changes after interfering microinjections by observing levels of immunofluorescence. Sophisticated software designed for calcium ratioing will be used for their data acquisition and analysis.MSUM is a regional comprehensive university that is committed to providing high quality research opportunities for undergraduates. The equipment and training package proposed in this application will be used to enhance the research programs of Drs. Brisch, Chastain, Provost and Wallert. It will provide their students with an advanced level of training. These projects will involve 25-30 students per year. These students will present their work at local, regional and national meetings and publish their results in review journals. Nearly 70% of the students involved in these projects will be women, and our history suggests that approximately 80% of them will continue their education in graduate or professional schools. In addition, an even greater number of students would have exposure to this state of the art equipment, as it would also be used in a number of our upper division courses. Involving students in research and incorporating research-based exercises in our teaching laboratories enhances not only the student's education, but also the effectiveness and success of our university.

明尼苏达州立大学摩尔黑德分校（MSUM）的Ellen Brisch博士获得了一笔赠款，通过升级我们目前的奥林巴斯IX-70明场/相衬显微镜来建立我们的显微镜能力。这项拨款将使我们能够将细胞注射、图像捕获、图像分析和高质量的图形准备能力纳入我们的教学和研究。该设备将由PI共享；Ellen Brisch博士和三位合伙人；Chris Chastain博士，Joseph Provost博士和Mark Wallert博士。这笔拨款将资助的设备和仪器包括：一个细胞注射系统，额外的免疫荧光过滤器立方体和高质量的100倍物镜，一个软件包，它将使我们能够检测和量化pH值的细微变化，一个隔离表和电动舞台，最先进的Macintosh计算机用于图像分析和图形准备，以及显微镜培训车间。四名研究员均从事本科教学和培训工作。我们目前的目标是通过训练本科生使用最先进的设备，为他们提供最先进的教育，并将我们的研究项目提升到更高的水平。调查人员领导的研究项目各不相同。布里希博士的研究项目主要集中在细胞分裂过程中，关键细胞器线粒体的复制和分离是如何协调的。布里希博士计划对一组酵母细胞周期突变体（不能正确完成细胞分裂周期的酵母细胞）进行检查，方法是对线粒体进行染色，并用荧光显微镜观察它们。查斯坦博士的研究项目侧重于了解C4光合途径中的酶活性。查斯坦博士计划通过免疫定位研究来研究C4光合酶在C3植物中的分布。Drs。Wallert和Provost在他们的研究工作中进行了大量的合作。他们的研究项目主要集中在有丝分裂信号通路如何改变细胞内ph。他们计划通过微注射各种干扰剂来改变特定的细胞信号通路来进行细胞注射实验。他们计划通过观察免疫荧光水平来定量干预显微注射后是否存在细微的pH变化。为钙定量设计的复杂软件将用于他们的数据采集和分析。MSUM是一所区域性综合性大学，致力于为本科生提供高质量的研究机会。本申请书所提出的设备和培训包将用于加强博士的研究计划。布里希，查斯坦，普洛斯特和沃勒特。它将为他们的学生提供高级水平的培训。这些项目每年将涉及25-30名学生。这些学生将在地方、区域和国家会议上展示他们的工作，并在评论期刊上发表他们的成果。参与这些项目的近70%的学生将是女性，我们的历史表明，其中约80%的人将继续在研究生或专业学校接受教育。此外，更多的学生将有机会接触到这种最先进的设备，因为它也将用于我们的一些高年级课程。让学生参与研究，并在教学实验室中加入以研究为基础的练习，不仅能提高学生的教育水平，也能提高大学的效率和成功。