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In vivo Macroscopic Fluorescence Lifetime Molecular Optical Imaging

In vivo Macroscopic Fluorescence Lifetime Molecular Optical Imaging
体内宏观荧光寿命分子光学成像
批准号:
10277118
负责人:
Margarida Barroso
金额:
$19.04万
依托单位国家:
美国
项目类别:
财政年份:
2020
资助国家:
美国
项目状态:
已结题
起止时间:
2020-05-01 至 2023-05-31
关键词:
3-DimensionalAchievementAlgorithmsAnimalsAntibodiesAspartateBindingBiochemicalBiological AssayBloodCancer BiologyCell Surface ProteinsCitiesCleaved cellComplexCoupledDevelopmentDrug Delivery SystemsDrug ReceptorsDrug TargetingDyesERBB2 geneEpidermal Growth Factor ReceptorFingerprintFluorescenceFluorescence MicroscopyFluorescence Resonance Energy TransferFunctional ImagingFundingGoalsHumanImageImage-Guided SurgeryIn VitroLabelLengthLibrariesLightMMP9 geneMammary NeoplasmsMasksMeasuresMetabolicMicroscopyMolecularMonitorMonoclonal AntibodiesMulti-Drug ResistanceOncologyOptical TomographyParentsPeptide HydrolasesPharmaceutical PreparationsPharmacologic SubstancePlayPositioning AttributePropertyReportingResearchRoleSeriesSideSignal TransductionSiteStructureTechnologyTestingTherapeuticTherapeutic Monoclonal AntibodiesTimeTissuesToxic effectToxinTrastuzumabantibody engineeringanticancer researchbasecancer imagingclinical translationclinically relevantdeep learningdetection sensitivitydrug developmentfluorescence lifetime imagingimagerimaging modalityimaging platformimaging studyimprovedin vivoin vivo Modelin vivo imagingin vivo monitoringinnovationinterstitialmacromoleculemalignant breast neoplasmmetabolic imagingmolecular imagingmultiplexed imagingnew technologynew therapeutic targetnext generationnoveloptical imagingpersonalized medicinepre-clinicalpreclinical imagingprogramsquantitative imagingreceptorreceptor bindingresistance mechanismresponseserial imagingsuccesstargeted deliverytargeted imagingtargeted treatmenttechnological innovationtooltraffickingtumortumor microenvironmenttumor xenograft

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中文摘要
翻译
体内时间分辨宽视场分子光学层析成像(亲本R01)
英文摘要
In vivo Time-Resolved Wide-Field Molecular Optical Tomography (Parent R01) ABSTRACT There is still great need in better characterizing new targeted therapies in vivo, especially prior to clinical translation. In this regard, preclinical molecular imaging is a central tool in the targeted drug development pipeline. However, there is still a lack of integrated imaging platforms that can enable longitudinal (multiple time points) and spatially-resolved monitoring of complex fingerprints including molecular, metabolic and functional signatures in the same tumor/subject. This new integrated multiplexing imaging platform will play a crucial role in the development of the next generation of targeted drugs and elucidating (multi-) drug resistance mechanisms. Recently, we have demonstrated the unique capabilities of optical imaging in quantifying receptor-target engagement in live subjects by leveraging fluorescence lifetime. This outstanding achievement was realized thanks to the combination of instrumental, algorithmic and biochemical innovations to enable, for the first time, whole-body time-resolved optical imaging based on structured light for 2D or 3D Förster resonance energy transfer (FRET) imaging in live subjects. Herein, using the RFA-CA-20-021, Revision Applications for Incorporation of NCI-Supported Technology to Accelerate Cancer Research, we propose to expand on the parent R01 and employ the IMAT-funded meditope antibody technology to quantitatively monitor in vivo and non- invasively the efficacy of HER2 receptor-targeted drug delivery systems using macroscopy fluorescence lifetime imaging (MFLI)-FRET imaging. We will establish and optimize an antibody-to-target imaging pipeline to quantitatively monitor the binding of near-infrared labeled anti-HER2 meditope antibodies to their respective targets (target engagement) in heterogeneous breast tumor microenvironments.
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AI enhanced lifetime-based mesoscopic in vivo imaging of tissue molecular heterogeneity
  • 批准号:
    10585510
  • 项目类别:
  • 资助金额:
    $66.08万
  • 财政年份:
    2023
  • 负责人:
    Margarida Barroso
  • 依托单位:
Artificial intelligence enhanced cancer cell classification based organelle morphology and topology
  • 批准号:
    10528867
  • 项目类别:
  • 资助金额:
    $23.02万
  • 财政年份:
    2022
  • 负责人:
    Margarida Barroso
  • 依托单位:
IMAT-ITCR Collaboration: Artificial intelligence enhanced breast cancer dormancy cell classification-based organelle-morphology and topology
  • 批准号:
    10884759
  • 项目类别:
  • 资助金额:
    $8.15万
  • 财政年份:
    2022
  • 负责人:
    Margarida Barroso
  • 依托单位:
In vivo Macroscopic Fluorescence Lifetime Molecular Optical Imaging
  • 批准号:
    10474962
  • 项目类别:
  • 资助金额:
    $79.35万
  • 财政年份:
    2020
  • 负责人:
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  • 依托单位:
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