In vivo Macroscopic Fluorescence Lifetime Molecular Optical Imaging

体内宏观荧光寿命分子光学成像

• 批准号: 10621919
• 负责人: Margarida Barroso
• 金额: $ 62.18万
• 依托单位: RENSSELAER POLYTECHNIC INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10621919
• 关键词: 3-Dimensional; Achievement; Algorithms; Animals; Biochemical; Biological; Biological Assay; Blood Vessels; Cancer Biology; Cells; Collaborations; Collection; Complex; Darkness; Data; Dedications; Detection; Development; Drug Delivery Systems; Drug Targeting; Drug resistance; ERBB2 gene; Electronics; Epidermal Growth Factor Receptor; Family member; Fingerprint; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Foundations; Functional Imaging; Goals; Hemoglobin; Human; Image; Image-Guided Surgery; Imaging ligands; Immunohistochemistry; Light; Longitudinal Studies; Malignant Neoplasms; Mammary Neoplasms; Measures; Metabolic; Methodology; Methods; Molecular; Molecular Probes; Molecular Target; Monitor; Optical Tomography; Optics; Oxygen; Penetration; Performance; Pharmaceutical Preparations; Photons; Physiological; Play; Productivity; Reporter Genes; Reporting; Research; Research Personnel; Resolution; Role; Side; Signal Transduction; Structure; Techniques; Therapeutic; Time; Tissues; bioimaging; clinical translation; clinically relevant; cost effective; deep learning; design; detector; drug development; drug efficacy; fluorescence imaging; fluorescence lifetime imaging; functional status; imager; imaging agent; imaging modality; imaging platform; imaging study; improved; in vivo; innovation; metabolic imaging; molecular imaging; multiplexed imaging; new technology; new therapeutic target; next generation; novel; optical imaging; personalized medicine; portability; pre-clinical; preclinical imaging; preclinical study; programs; quantitative imaging; receptor; resistance mechanism; response; serial imaging; targeted treatment; technological innovation; tomography; tool; treatment response; tumor; tumor xenograft; user-friendly

In vivo Macroscopic Fluorescence Lifetime Molecular Optical Imaging ABSTRACT There is still great need in better characterizing new targeted therapies in vivo, especially prior to clinical translation. In this regard, preclinical molecular imaging is a central tool in the targeted drug development pipeline. However, there is still a lack of integrated imaging platforms that can enable longitudinal (multiple time points) and spatially-resolved monitoring of complex fingerprints including molecular, metabolic and functional signatures in the same tumor/subject. This new integrated multiplexing imaging platform will play a crucial role in the development of the next generation of targeted drugs and elucidating (multi-) drug resistance mechanisms. Recently, we have demonstrated the unique capabilities of optical imaging in quantifying receptor-target engagement in live subjects by leveraging fluorescence lifetime. This outstanding achievement was realized thanks to the combination of instrumental, algorithmic and biochemical innovations to enable, for the first time, whole-body time-resolved optical imaging based on structured light for 2D or 3D Förster resonance energy transfer (FRET) imaging in live subjects. Herein, we will further impact the field of optical preclinical imaging and drug delivery assessment by 1) integrating a cutting-edge high-resolution, time-resolved SPAD array imager for improved photon collection efficiency, spatial resolution, and portability; 2) we will harness the latest developments in Deep Learning (DL) for ultra-fast, quantitative, but fitting/iterative inverse solver-free image formation, in both 2D and 3D providing image formation/processing solutions to facilitate multiplexed imaging; 3) we will implement new functionalities in our imaging platform to enable the concurrent longitudinal imaging of multiple clinically relevant target-receptor interactions (e.g. HER receptor family members involved in many cancers) as well as metabolic and functional status across the whole-tumor region in live intact animals.

体内宏观荧光寿命分子光学成像

项目成果

Roadmap on Deep Learning for Microscopy.

显微镜深度学习路线图。

• 发表时间: 2023
• 期刊: ArXiv
• 作者: Volpe,Giovanni;Wählby,Carolina;Tian,Lei;Hecht,Michael;Yakimovich,Artur;Monakhova,Kristina;Waller,Laura;Sbalzarini,IvoF;Metzler,ChristopherA;Xie,Mingyang;Zhang,Kevin;Lenton,IsaacCD;Rubinsztein-Dunlop,Halina;Brunner,Daniel;Ba
• 通讯作者: Ba

Contrast-enhanced photon-counting micro-CT of tumor xenograft models.

肿瘤异种移植模型的对比增强光子计数显微 CT。

• DOI: 10.1101/2024.01.03.574097
• 发表时间: 2024
• 期刊: bioRxiv : the preprint server for biology
• 作者: Li,Mengzhou;Guo,Xiaodong;Verma,Amit;Rudkouskaya,Alena;McKenna,AntigoneM;Intes,Xavier;Wang,Ge;Barroso,Margarida
• 通讯作者: Barroso,Margarida

NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera.

使用新型时间选通 SPAD 相机进行近红外荧光寿命宏观成像。

• DOI: 10.1117/12.2649227
• 发表时间: 2023
• 期刊: Proceedings of SPIE--the International Society for Optical Engineering
• 作者: Michalet,Xavier;Ulku,ArinC;Wayne,MichaelA;Weiss,Shimon;Bruschini,Claudio;Charbon,Edoardo
• 通讯作者: Charbon,Edoardo