2001 TSE: NSF/EPA Novel Self-Assembling Interfacial Biocatalysis in Organic-Aqueous Biphasic Systems for Environmentally Benign Chemical Processing

2001 TSE：NSF/EPA 新型有机-水双相系统中的自组装界面生物催化，用于环境友好的化学加工

• 批准号: 0124769
• 负责人: Ping Wang
• 金额: $ 10万
• 依托单位: University of Akron
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0124769&HistoricalAwards=false
• 关键词: 2001; TSE; NSF; EPA; Novel

The objective of this project is to functionalize water-soluble enzymes to provide hydrophobic and hydrophilic parts, which are expected to self-assemble and catalyze reactions at organic-aqueous interfaces. Current methodologies with enzymes placed in either aqueous or organic solutions suffer significant solubility and mass transfer limitations for reacting systems. The proposed interfacial configuration provides reagents in the biphasic systems the maximum access to the biocatalyst so that solubility and mass transfer limitations can be reduced. The polymeric modifying groups will alter the microenvironmental interactions around the enzyme moieties and reduce the interfacial tension, and thus affect the interfacial assembly and activation of enzymes. Fundamentals related to the interfacial assembly and reaction kinetics, particularly the effect of interfacial tension, will be explored both experimentally and theoretically. The feasibility of this interfacial biocatalysis will be examined and demonstrated for the enzymatic production of epoxides and glycosides. These enzymatic syntheses may lead to more environmentally-friendly routes for large-scale production of valuable chemicals.

该项目的目标是功能化水溶性酶，以提供疏水和亲水部分，这些部分有望在有机-水界面自组装和催化反应。目前将酶置于水或有机溶液中的方法在反应系统中存在明显的溶解度和传质限制。所提出的界面配置为双相体系中的试剂提供了与生物催化剂的最大接触，从而降低了溶解度和传质限制。高分子修饰基团会改变酶周围的微环境相互作用，降低界面张力，从而影响酶的界面组装和活化。与界面组装和反应动力学相关的基本原理，特别是界面张力的影响，将在实验和理论上进行探讨。这种界面生物催化的可行性将被用于环氧化物和糖苷的酶促生产。这些酶促合成可能会为大规模生产有价值的化学品带来更环保的途径。