Purchase of a MALDI-TOF Mass Spectrometer

购买 MALDI-TOF 质谱仪

• 批准号: 0131222
• 负责人: J. Devens Gust
• 金额: $ 22.6万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2005-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0131222&HistoricalAwards=false
• 关键词: Purchase; MALDI; TOF; Mass; Spectrometer

With support from the Chemistry Research Instrumentation and Facilities (CRIF) Program, the Department of Chemistry and Biochemistry at Arizona State University will acquire a Matrix-assisted Laser Desorption Ionization - Time of Flight (MALDI-TOF) Mass Spectrometer. This instrument will facilitate research in a number of areas: a) the synthesis of organic compounds, including large porphyrin-based complexes; b) characterization of intermediates and products involved in the biosynthesis of the glycosylated carotenoid myxoxanthophyll; c) characterization of proteins that have undergone posttranslational modifications; d) characterization of synthetic DNA binding polypeptides; and e) proteomics study on extracellular enzymes that degrade phenolic comopounds. Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry is the technique of choice for obtaining accurate molecular weights on molecules up to and over 300,000 daltons, with extremely high sensitivity. Use of a MALDI-TOF mass spectrometer has therefore become a standard technique in most DNA/protein core facilities. The ability to obtain very accurate molecular weights of biomolecules is fundamental to all biological and biomedical research and essential in a modern research and training environment.

在化学研究仪器和设施（CRIF）计划的支持下，亚利桑那州立大学化学和生物化学系将获得一台基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱仪。该仪器将促进若干领域的研究：a)有机化合物的合成，包括大型卟啉基配合物；B)糖基化类胡萝卜素粘叶黄素生物合成的中间体和产物的表征；C)翻译后修饰蛋白的表征；d)合成DNA结合多肽的表征；e)降解酚类化合物的胞外酶的蛋白质组学研究。矩阵辅助激光解吸电离（MALDI）飞行时间（TOF）质谱法是获得精确分子质量的技术选择高达和超过300,000道尔顿，具有极高的灵敏度。因此，使用MALDI-TOF质谱仪已成为大多数DNA/蛋白质核心设施的标准技术。获得非常精确的生物分子分子量的能力是所有生物和生物医学研究的基础，也是现代研究和培训环境中必不可少的。