Molecular Dynamics in Tumor Cell Extravasation

在这项研究者发起的奖励下，该项目将重点评估人中性粒细胞/白细胞（PMN）粘附黑色素瘤细胞对黑色素瘤细胞粘附和跨内皮转运的影响，即肿瘤细胞外渗。有证据表明，白细胞有助于肿瘤细胞的粘附和迁移。研究将在一种新的流动室中进行，该室允许检查流动对肿瘤细胞附着的影响。四个目标是：1)表征肿瘤诱导的白细胞在动态肿瘤细胞外渗中的作用；2)鉴定与PMN募集相关的肿瘤分泌趋化因子，并确定它们在流动条件下协助肿瘤外渗的能力；3)确定流体动力学剪切对肿瘤-白细胞相互作用中粘附效率的影响；4)通过将工程原理应用于细胞生物学来促进教育/教学。

0138474DongUnder this Investigator Initiated Award, the PI will focus on assessing the effect that human neutrophil/leukocyte (PMN) adhesion to melanoma cells has on melanoma cell adhesion and transmigration across endothelium, i.e., tumor cell extravasation. Evidence suggests that leukocytes assist tumor cell adhesion and transmigration. Studies will be performed in a novel flow chamber that permits examination of the effect of flow on tumor cell attachment. The four aims are: 1) to characterize the role of tumor-elicited leukocytes in dynamic tumor cell extravasation, 2) to identify tumor-secreted chemokines associated with PMN recruitment and determine their ability to assist with tumor extravasation under flow conditions, 3) to determine hydrodynamic shear effect on adhesion efficiency in tumor-leukocyte interaction and 4) to foster education/teaching through the application of engineering principles to cell biology.