Noncovalent, Near-Infrared Labels as Facilitators of Protein Determination by Capillary Electrophoresis

非共价近红外标记作为毛细管电泳蛋白质测定的促进剂

• 批准号: 0138963
• 负责人: Christa Colyer
• 金额: $ 20.14万
• 依托单位: Wake Forest University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0138963&HistoricalAwards=false
• 关键词: Noncovalent; Near; Infrared; Labels; Facilitators

Professor Christa Colyer of Wake Forest University is supported by the Analytical and Surface Chemistry Program to perform studies on the use of noncovalent dye labels for protein detection by Laser Induced Fluorescence (LIF) after seperation by capillary electrophoresis (CE). LIF detection requires labeling of proteins with fluorescent dyes. The proposed work aims to label proteins noncovalently using dyes that are excited by near infrared photons, allowing the eventual use of cheap laser diodes aboard microchip CE. Seperation and quantitation of proteins, epecially from ultrasmall volumes, represents a particular challenge in analytical chemistry. CE requires only nanoliters of sample and so is appropriate for investigating the fluids of single cells. The methods are of great use in the investigation of proteomics.

维克森林大学的Christa Colyer教授在分析和表面化学项目的支持下，对毛细管电泳（CE）分离后激光诱导荧光（LIF）检测蛋白质使用非共价染料标签进行了研究。LIF检测需要用荧光染料标记蛋白质。这项提议的工作旨在使用近红外光子激发的染料来标记蛋白质的非共价，从而最终允许在微芯片CE上使用廉价的激光二极管。蛋白质的分离和定量，特别是来自超小体积的蛋白质，是分析化学中的一个特殊挑战。CE只需要纳升的样品，因此适用于研究单个细胞的液体。该方法在蛋白质组学研究中具有重要的应用价值。