Acquisition of a quadrupole time-of-flight mass spectrometer

购买四极杆飞行时间质谱仪

• 批准号: 0947028
• 负责人: Christa Colyer
• 金额: $ 44.21万
• 依托单位: Wake Forest University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0947028&HistoricalAwards=false
• 关键词: Acquisition; quadrupole; time; flight; mass

With this award from the Chemistry Research Instrumentation and Facilities: Departmental Multi-User Instrumentation program (CRIF:MU), Professors Christa L. Colyer and Rebecca W. Alexander will acquire a quadrupole time-of-flight (Q-TOF) mass spectrometer (MS). The instrument will be used to support research in the following areas: 1) affinity-based capillary electrophoresis studies to facilitate bioprobe design and microbe detection; 2) dissecting inter-domain communication in methionyl-tRNA synthetase; 3) excited state energy transfer amongst natural products; 4) structures and redox chemistry in sulfinic acid reduction; 5) modeling biological networks in arabidopsis through integration of genomic, proteomic, and metabolomic data; and, 6) cysteine sulfenic acids in catalysis and regulation.Mass spectrometry (MS) is used to identify the chemical composition of a sample and determine its purity by measuring the mass of the molecular constituents in the sample after they are ionized and detected by the mass spectrometer. Mass spectrometry is one of the most widely used analytical tools by chemists and biochemists. The acquisition will provide training for undergraduate and graduate students in this technique using modern instrumentation through research programs in the department and in the undergraduate chemistry laboratories.

凭借化学研究仪器和设施：部门多用户仪器计划 (CRIF:MU) 的这一奖项，Christa L. Colyer 和 Rebecca W. Alexander 教授将获得一台四极杆飞行时间 (Q-TOF) 质谱仪 (MS)。 该仪器将用于支持以下领域的研究：1）基于亲和力的毛细管电泳研究，以促进生物探针设计和微生物检测； 2) 剖析甲硫氨酰-tRNA合成酶中的域间通讯； 3）天然产物之间的激发态能量转移； 4）亚磺酸还原中的结构和氧化还原化学； 5) 通过整合基因组、蛋白质组和代谢组数据对拟南芥生物网络进行建模； 6）半胱氨酸磺酸在催化和调节中的作用。质谱（MS）用于鉴定样品的化学成分并通过测量样品中分子成分电离并经质谱仪检测后的质量来确定其纯度。 质谱是化学家和生物化学家最广泛使用的分析工具之一。 此次收购将通过该系和本科生化学实验室的研究项目，使用现代仪器为本科生和研究生提供该技术的培训。