Affinity-based CE Studies to Facilitate Bioprobe Design and Microbe Detection.

基于亲和力的 CE 研究促进生物探针设计和微生物检测。

• 批准号: 0809756
• 负责人: Christa Colyer
• 金额: $ 38.6万
• 依托单位: Wake Forest University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0809756&HistoricalAwards=false
• 关键词: Affinity; based; CE; Studies; Facilitate

The Analytical and Surface Chemistry Program in the Division of Chemistry supports Prof. Christa Colyer and her group at Wake Forest University to develop rapid and sensitive assays based on capillary electrophoresis (CE) methodologies and employing rationally designed bioprobes for protein targets. Applications include quantitative assessment of the noncovalent binding properties of novel squarylium and triazolylcoumarin dyes towards proteins based on CE affinity, and comparison with solid phase microextraction methods (in collaboration with Dr. Hiroyuki Nakazumi of Osaka Prefecture University in Japan; David Silcott of S3I, LLC; and Dr. Janusz Pawliszyn of the University of Waterloo, Canada). Application of affinity CE to ligand-receptor interactions essential to the function of the B7 family of proteins involved in the regulation of immune responses is also being assessed (in collaboration with Dr. Lieping Chen of Johns Hopkins University). CE-based assays employing designed boronic acid-based bioprobes are being developed (in collaboration with Dr. Qian Wang and Dr. Karen Fox of the University of South Carolina) to identify carbohydrate-based residues unique to the surfaces of viral and bacterial agents of importance to human health and security, such as the influenza virus and Bacillus anthracis (anthrax) spores. Improved bacterial analysis will support differentiation and quantitation of various intact cyanobacteria species, their constituent phycobiliproteins, and their tetrapyrrole bilin pigments. This unique three-pronged CE methodology should enhance understanding of the role of cyanobacteria in the global carbon cycle.In conjunction with this research, Dr. Colyer is engaged in various community and academic outreach ventures, such as a summer research program for undergraduate students at High Point University and the creation of a workshop entitled "Electrophoresis Working Weekend" for the academic enrichment of faculty and students from regional colleges and universities. An international student exchange between Wake Forest University and Osaka Prefecture University in Japan will better prepare students for the increasingly interdisciplinary and globalized nature of scientific research. Additionally, a series of case studies based on research results will be developed to provide college-level instructors with the resources necessary to teach modern separation science and biosensing methods. This series of case studies, to be called "Separation Shorts," will be submitted for publication in the peer-reviewed collection of the NSF-funded National Center for Case Study Teaching in Science.

化学学部的分析和表面化学项目支持维克森林大学的Christa Colyer教授和她的团队开发基于毛细管电泳（CE）方法的快速和敏感的检测方法，并使用合理设计的生物探针来检测蛋白质目标。应用包括基于CE亲和力的新型方英和三唑基香豆素染料对蛋白质的非共价结合特性的定量评估，以及与固相微萃取方法的比较（与日本大阪市立大学的Hiroyuki Nakazumi博士、sgi有限责任公司的David Silcott博士和加拿大滑铁卢大学的Janusz Pawliszyn博士合作）。与约翰霍普金斯大学的Lieping Chen博士合作，还在评估亲和性CE在B7蛋白家族参与免疫应答调节的功能所必需的配体-受体相互作用中的应用。与南卡罗莱纳大学的王茜博士和卡伦·福克斯博士合作，正在开发采用设计的硼酸生物探针的基于ce的检测方法，以识别对人类健康和安全至关重要的病毒和细菌病原体表面特有的碳水化合物基残留物，如流感病毒和炭疽芽孢杆菌孢子。改进的细菌分析将支持各种完整蓝藻物种的分化和定量，它们的组成藻胆蛋白，以及它们的四吡啶胆色素。这种独特的三管齐下的CE方法应该加强对蓝藻在全球碳循环中的作用的理解。在这项研究的同时，Colyer博士还参与了各种社区和学术推广活动，例如为High Point大学的本科生开展的夏季研究项目，以及为地区高校的教师和学生创建的名为“电泳工作周末”的研讨会。维克森林大学和日本大阪府立大学之间的国际学生交流将使学生更好地为日益跨学科和全球化的科学研究做好准备。此外，一系列基于研究结果的案例研究将被开发出来，为大学水平的教师提供必要的资源来教授现代分离科学和生物传感方法。这一系列的案例研究，被称为“分离短片”，将被提交到nsf资助的国家科学案例研究教学中心的同行评议文集中发表。