A Digitial Confocal Microscope and Image Analysis System for Cell Biology

用于细胞生物学的数字共焦显微镜和图像分析系统

• 批准号: 0140676
• 负责人: Thomas Shea
• 金额: $ 6.48万
• 依托单位: University of Massachusetts Lowell
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0140676&HistoricalAwards=false
• 关键词: Digitial; Confocal; Microscope; Image; Analysis

This award supports the purchase of a digital confocal microscope and image analysis system. The image analysis system will allow custom-design of unlimited programming routines for automated and semi-automated image capture for conventional, confocal and real-time video microscopy. The confocal system will employ an inverted microscope to accommodate imaging of live cell cultures, whole tissue mounts and fixed, slide-mounted samples. The group of 5 major users who will share the instrument have research programs in diverse aspects of cell biology, including neurobiology, cytoskeletal dynamics, cell-cell interactions, and environment interactions between bacteria and algae. Confocal microscopy has become a standard tool in cell biology. However, there is currently no confocal microscope at or near the institution. Thus the impact on the users' research will be substantial. In addition, over 20 students at all levels from undergraduate to postdoctoral have ongoing projects that will directly benefit from the confocal system.

该奖项支持购买数字共焦显微镜和图像分析系统。图像分析系统将允许定制设计的无限编程例程的自动化和半自动化图像捕获的传统，共焦和实时视频显微镜。共聚焦系统将采用倒置显微镜，以适应活细胞培养物、全组织标本和固定载玻片标本的成像。 将共享该仪器的5个主要用户组在细胞生物学的各个方面都有研究计划，包括神经生物学，细胞骨架动力学，细胞-细胞相互作用以及细菌和藻类之间的环境相互作用。 共聚焦显微镜已成为细胞生物学中的标准工具。 然而，目前在该机构或其附近没有共聚焦显微镜。 因此，对用户研究的影响将是巨大的。 此外，从本科到博士后的各个层次的20多名学生正在进行的项目将直接受益于共聚焦系统。