NIRT: Bioavailability of Aromatic Hydrocarbons and Dynamics of Their Interactions with Humic and Fulvic Acids: Linking Molecular- and Microbial-Scale Interactions

该提案是根据纳米科学与工程计划NSF 01-157（类别NIRT）收到的。该项目将在纳米尺度上确定关键的物理、化学和生物条件，这些条件控制着从土壤、沉积物和垃圾填埋场分离出来的与NOM相关的芳香烃的生物可利用性。在对NOM进行表征和定量后，将评估局部分子尺度相互作用、烃类残留百分比和相互作用活化能对生物利用度的影响。萘双加氧酶活性与NOM类型、浓度和NOM芳香老化有关。耦合微生物的生物利用度将使用生物发光报告。该研究的一个结果将是基于核磁共振的特征的发展，这将与酶测定和微生物生物测定相关。

0210839 Nanny This proposal was received in response to the Nanoscale Science and Engineering Initiative NSF 01-157, category NIRT. The project will identify key physical, chemical and biological conditions at the nano-scale that govern the bioavailability of aromatic hydrocarbons associated with NOM isolated from soils, sediments, and landfills. After NOM characterization and quantitation, the impact of the localized molecular scale interactions, % hydrocarbon residue, and activation energy of interactions will be assessed with respect to bioavailability. Naphthalene dioxygenase activity will be characterized as a function of NOM type and concentration and NOM-aromatic aging. Coupling to microbial bioavailability will be made using bioluminescence reporting. An outcome from the research will be the development of NMR based characterizations that will correlate with an enzymatic assay and a microbial bioassay.