From Proplastid to Chloroplast: Understanding Plastid Differentiation in Maize through Microarray and Proteome Analysis

从原生质体到叶绿体:通过微阵列和蛋白质组分析了解玉米质体分化

基本信息

项目摘要

Although best known for their role in photosynthesis, plastids perform several essential functions, which include synthesis of hormones, amino acids, fatty acids, lipids, vitamins, nucleotides and secondary metabolites. Despite their essential role, little is known of the developmental signals that help drive the process of plastid differentiation.. To address this important agricultural and biological problem, we will take advantage of new tools that have been developed for genomic and proteomic studies. The availability of extensive plant genome sequences has enabled us to identify a set of approximately 1500 maize EST sequences that encode chloroplast targeted proteins. Through array profiling, we will examine the expression of these genes in the two photosynthetic cell types in maize, the bundle sheath and mesophyll. In parallel, we will initiate a broad survey of the estimated 3,000 proteins predicted to accumulate in plastids using highly sensitive mass spectroscopy techniques. The bioinformatic integration of proteomic and expression profiling data will provide a detailed picture of transcription and translational control mechanisms utilized by the maize photosynthetic plastids. Finally, transgenic plants carrying bundle sheath and mesophyll cell-specific markers will be constructed to help monitor the differentiation process. These GFP constructs will permit a detailed analysis of bundle sheath and mesophyll cell biology and facilitate the analysis of a series of maize plastid mutants. Several results are expected from this project, such as an improved understanding of the biochemical functions of the different plastid types and novel insights into the differentiation process. As several chloroplast functions are directly related to the agronomic performance of the maize plant, these studies will greatly improve our understanding and ability to engineer this important crop plant.
虽然最著名的是在光合作用中的作用,但叶绿体执行几个基本功能,包括合成激素、氨基酸、脂肪酸、脂类、维生素、核苷酸和次生代谢物。尽管它们发挥着重要的作用,但人们对帮助驱动叶绿体分化过程的发育信号知之甚少。为了解决这一重要的农业和生物学问题,我们将利用已经开发的基因组和蛋白质组研究的新工具。广泛的植物基因组序列的可获得性使我们能够识别一组大约1500个玉米EST序列,这些序列编码叶绿体靶标蛋白。通过阵列图谱,我们将检测这些基因在玉米的两种光合作用细胞类型--束鞘和叶肉中的表达。与此同时,我们将利用高灵敏的质谱学技术,对预计在叶绿体中积累的约3,000种蛋白质进行广泛调查。蛋白质组和表达谱数据的生物信息学整合将提供玉米光合体所利用的转录和翻译调控机制的详细图景。最后,将构建携带束鞘和叶肉细胞特异性标记的转基因植物,以帮助监测分化过程。这些GFP结构将允许对束鞘和叶肉细胞生物学进行详细分析,并有助于分析一系列玉米叶绿体突变体。这个项目预计会有几个结果,比如对不同类型的叶绿体的生化功能的更好的理解,以及对分化过程的新见解。由于叶绿体的几个功能与玉米植株的农艺表现直接相关,这些研究将极大地提高我们对这一重要作物的理解和工程能力。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Klaas van Wijk其他文献

GFS9はシロイヌナズナの暗所芽生えにおけるプラスチドのピースミールオートファジーに関与する
GFS9 参与拟南芥黑暗出芽期间的质体和平粉自噬。
  • DOI:
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    0
  • 作者:
    石田宏幸;石田ひろみ;泉 正範;林 誠;牧野 周;Klaas van Wijk
  • 通讯作者:
    Klaas van Wijk

Klaas van Wijk的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Klaas van Wijk', 18)}}的其他基金

Conference: 2023 Chloroplast Biotechnology GRC & GRS: Harnessing the SynBio Revolution for Metabolic Engineering and Enhanced Photosynthesis
会议:2023年叶绿体生物技术GRC
  • 批准号:
    2243932
  • 财政年份:
    2023
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Standard Grant
DETERMINATION OF THE N-DEGRON PATHWAY AND ITS SUBSTRATES IN PLANT CHLOROPLASTS
植物叶绿体中N-DEGRON途径及其底物的测定
  • 批准号:
    2322813
  • 财政年份:
    2023
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Standard Grant
Conference: 2023 Plant Proteolysis Gordon Research Conference
会议:2023年植物蛋白水解戈登研究会议
  • 批准号:
    2309281
  • 财政年份:
    2023
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Standard Grant
Tools4Cells:EAGER: CRYO-EM ANALYSIS OF THE CHLOROPLAST CLP PROTEASE SYSTEM THROUGH AFFINITY PURIFICATION OF ENDOGENOUS COMPLEXES
Tools4Cells:EAGER:通过内源复合物的亲和纯化对叶绿体 CLP 蛋白酶系统进行冷冻电镜分析
  • 批准号:
    2222495
  • 财政年份:
    2022
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Standard Grant
CLPS1-CLPF ADAPTORS FOR PROTEASE SUBSTRATE SELECTION IN CHLOROPLASTS
用于叶绿体中蛋白酶底物选择的 CLPS1-CLPF 接头
  • 批准号:
    1940961
  • 财政年份:
    2020
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Standard Grant
TRTech-PGR: A PeptideAtlas for Arabidopsis thaliana and other plant species; harnessing world-wide proteomics data and mining for biological features
TRTech-PGR:拟南芥和其他植物物种的肽图谱;
  • 批准号:
    1922871
  • 财政年份:
    2019
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Standard Grant
EAGER: Quantitative reporter systems for in vivo testing of an N-end rule for protein stability in plant chloroplasts
EAGER:用于体内测试植物叶绿体中蛋白质稳定性 N 端规则的定量报告系统
  • 批准号:
    1834636
  • 财政年份:
    2018
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Standard Grant
CHLOROPLAST SOLUBLE PROTEASES AND THEIR PHYSIOLOGICAL SUBSTRATES: An integrated genetic and targeted systems analysis of chloroplast proteolysis
叶绿体可溶性蛋白酶及其生理底物:叶绿体蛋白水解的综合遗传和靶向系统分析
  • 批准号:
    1614629
  • 财政年份:
    2016
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Standard Grant
2016 Mitochondria and Chloroplasts; Evolution, Biogenesis and Quality Control GRC
2016 线粒体和叶绿体;
  • 批准号:
    1620533
  • 财政年份:
    2016
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Standard Grant
Selection and Delivery of Substrates to the Essential Clp Protease Complex in Plastids of Arabidopsis Thaliana
拟南芥质体中必需 Clp 蛋白酶复合物的底物选择和递送
  • 批准号:
    1021963
  • 财政年份:
    2010
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Continuing Grant

相似国自然基金

CIA1 (Chloroplast Import Apparatus 1)调控拟南芥营养生长阶段转变的分子机理
  • 批准号:
    31300997
  • 批准年份:
    2013
  • 资助金额:
    22.0 万元
  • 项目类别:
    青年科学基金项目

相似海外基金

Defining the molecular basis of chloroplast transcription of photosynthetic genes
定义光合基因叶绿体转录的分子基础
  • 批准号:
    BB/Y003802/1
  • 财政年份:
    2024
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Research Grant
A powerful directed-evolution tool for exploitation of chloroplast engineering biology
用于叶绿体工程生物学开发的强大定向进化工具
  • 批准号:
    BB/Y008162/1
  • 财政年份:
    2024
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Research Grant
Structure and function of the chloroplast transcription machinery
叶绿体转录机制的结构和功能
  • 批准号:
    MR/X033481/1
  • 财政年份:
    2024
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Fellowship
Conference: 2023 Chloroplast Biotechnology GRC & GRS: Harnessing the SynBio Revolution for Metabolic Engineering and Enhanced Photosynthesis
会议:2023年叶绿体生物技术GRC
  • 批准号:
    2243932
  • 财政年份:
    2023
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Standard Grant
A study on division of photosynthetic metabolism associated with the uneven distribution of Rubisco and starch in the chloroplast stromal space
叶绿体基质空间中Rubisco和淀粉分布不均与光合代谢划分的研究
  • 批准号:
    23H02195
  • 财政年份:
    2023
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular analysis of nuclear factors governing the morphology and segregation chloroplast DNA
控制叶绿体 DNA 形态和分离的核因素的分子分析
  • 批准号:
    23H02509
  • 财政年份:
    2023
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Elucidation on a transcriptional and post-transcriptional regulation mechanism of the diurnal expression rhythm of chloroplast genes.
阐明叶绿体基因昼夜表达节律的转录和转录后调控机制。
  • 批准号:
    23K05146
  • 财政年份:
    2023
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Rotation 1: Validation of a putative MYB transcription factor involved in chloroplast development
第 1 轮:验证参与叶绿体发育的推定 MYB 转录因子
  • 批准号:
    2887717
  • 财政年份:
    2023
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Studentship
Functional analysis of THIOL, a novel chloroplast division protein with different roles.
具有不同作用的新型叶绿体分裂蛋白THIOL的功能分析。
  • 批准号:
    23K14210
  • 财政年份:
    2023
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Getting the green light: How chloroplast gene expression is activated by light
获得批准:叶绿体基因表达如何被光激活
  • 批准号:
    2869544
  • 财政年份:
    2023
  • 资助金额:
    $ 382.76万
  • 项目类别:
    Studentship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了