QuBIC: Molecular Robotics for DNA Nanostructures

EIA-0218359浩言杜克大学DNA纳米结构的分子机器人这个项目的目标是开发分子马达，并将其整合到自组装的DNA晶格中。该项目的主要目标将是开发构建新型DNA马达的实验概念验证演示，例如设计为同时具有平移和旋转运动的DNA马达。将分子马达结合到DNA阵列中有许多应用：它可以使用排列在DNA拼接阵列上的分子马达设备来选择性地操纵分子；DNA马达阵列可以提供一种机制来对元素(瓦片)保持状态的阵列进行DNA计算；并行细胞自动机计算可以从每个保持状态的有限状态自动机阵列执行。该项目还在开发包含马达的DNA纳米结构，这些马达可以在不改变环境的情况下自动运行。目前正在测试使用“燃料DNA”来提供能量来驱动DNA纳米结构运动的方法。作为另一种方法，实验是将激动素等蛋白质马达整合到DNA晶格中。特别是，将测试使用选择性适配子结合将蛋白质发动机连接到DNA晶格的周期性位置。由此产生的蛋白质马达阵列在纳米机器人学中有许多应用，例如，它们可能对纳米颗粒的分类和运输非常有用。

EIA-0218359Hao Yan Duke UniversityMolecular Robotics for DNA NanostructuresThe objective of this project is to develop molecular motors that are incorporated into self-assembled DNA lattices. The main goal of this project will be to develop experimental proof-of concept demonstrations of the construction of novel DNA motors such as a DNA motor that is designed to have both translational and rotational motion. Incorporation of molecular motors into DNA arrays has many applications: It can selectively manipulate molecules using molecular motor devices arranged on DNA tiling arrays; A DNA array of motors may offer a mechanism to do DNA computation of arrays whose elements (the tiles) hold state; Parallel cellular automata computation may be executed from arrays of finite state automata each of which hold state. This project is also developing DNA nanostructures containing motors that operate autonomously without environmental changes. Methods are being tested to use "fuel DNA" to provide energy to drive the motion of DNA nanostructures. As an alternative approach, experiments are conducted to incorporate protein motors such as Kinesin into DNA lattices. In particular, the use of selective aptamer binding to link protein motors to periodic sites of a DNA lattice will be tested. The resulting arrays of protein motors have many applications to nanorobotics, e.g., they are potentially very useful for sorting and transport of nanoparticles.