QuBIC: Molecular Robotics for DNA Nanostructures

EIA-0218359Hao Yan 杜克大学 DNA 纳米结构分子机器人该项目的目标是开发纳入自组装 DNA 晶格的分子马达。该项目的主要目标是开发新型 DNA 电机构造的实验概念验证演示，例如设计为具有平移和旋转运动的 DNA 电机。将分子马达纳入DNA阵列有许多应用：它可以利用排列在DNA平铺阵列上的分子马达装置选择性地操纵分子；电机的 DNA 阵列可以提供一种机制来对其元素（图块）保持状态的阵列进行 DNA 计算；并行元胞自动机计算可以从有限状态自动机的阵列执行，每个有限状态自动机都保持状态。该项目还正在开发包含电机的 DNA 纳米结构，该电机可以在不改变环境的情况下自主运行。正在测试使用“燃料 DNA”提供能量来驱动 DNA 纳米结构运动的方法。作为一种替代方法，进行实验将驱动蛋白等蛋白质马达整合到 DNA 晶格中。特别是，将测试使用选择性适体结合将蛋白质马达连接到 DNA 晶格的周期性位点。由此产生的蛋白质马达阵列在纳米机器人领域有许多应用，例如，它们对于纳米粒子的分类和运输可能非常有用。

EIA-0218359Hao Yan Duke UniversityMolecular Robotics for DNA NanostructuresThe objective of this project is to develop molecular motors that are incorporated into self-assembled DNA lattices. The main goal of this project will be to develop experimental proof-of concept demonstrations of the construction of novel DNA motors such as a DNA motor that is designed to have both translational and rotational motion. Incorporation of molecular motors into DNA arrays has many applications: It can selectively manipulate molecules using molecular motor devices arranged on DNA tiling arrays; A DNA array of motors may offer a mechanism to do DNA computation of arrays whose elements (the tiles) hold state; Parallel cellular automata computation may be executed from arrays of finite state automata each of which hold state. This project is also developing DNA nanostructures containing motors that operate autonomously without environmental changes. Methods are being tested to use "fuel DNA" to provide energy to drive the motion of DNA nanostructures. As an alternative approach, experiments are conducted to incorporate protein motors such as Kinesin into DNA lattices. In particular, the use of selective aptamer binding to link protein motors to periodic sites of a DNA lattice will be tested. The resulting arrays of protein motors have many applications to nanorobotics, e.g., they are potentially very useful for sorting and transport of nanoparticles.