Collaborative Research: Development of a Combined in Situ Particle Imaging Velocimeter /Fluorescence Imaging System

PI建议建立一个新的系统，该系统将对荧光和散射光的多个波长进行成像，并进行立体粒子图像测速（3D PIV）测量。这些数据将使地图的流体流动和衍生的数量，如应变率，涡度，和耗散率的湍流动能被生产。测量将在相同的尺度（20 x 20 x 0.6 cm，分辨率为100 um）上进行，几乎同时进行，允许生物分布到微尺度物理流的原位3D映射。有人提出，多个成像波长将允许荧光颗粒之间的分类歧视的程度，而侧散射光将提供所有粒子的图像，散射的激发辐照度，包括大多数的浮游植物出现在荧光图像中，以及浮游动物。三维PIV多光谱成像荧光计将安装在一个自由落体平台上，该平台配有一套辅助仪器和一个水取样器，用于地面实况成像，该平台已经建造并显示，可提供水柱中浮游生物的未受干扰的视图。将在平台下方延伸的平面中捕获图像，以获得不受平台影响的分布。船上的落射荧光显微镜将用于确定图像中的主要生物体，由于缺乏技术，将浮游生物的微尺度分布与海洋中的微尺度物理动力学联系起来在很大程度上受到限制。多波长成像和PIV相结合，将允许在现场调查的荧光颗粒/散装荧光的关系，浮游生物的微尺度分布的微尺度流动，空间关系的食草动物和初级生产者，和物理机制的罚款和微尺度生物斑块。该仪器是由PI开发的现有2D成像荧光计的增强，该荧光计只有一个传感波长，并且不会同时收集与荧光图像相同尺度的物理数据。

The PIs propose to build a new system that will image multiple wavelengths of fluoresced and scattered light and perform stereo particle image velocimetry (3D PIV) measurements. These data will enable maps of fluid flow and derived quantities such as strain rate, vorticity, and the dissipation rate of turbulent kinetic energy to be produced. Measurements will be made on identical scales (20 x 20 x 0.6 cm with 100 um resolution), nearly simultaneously, allowing in situ 3D mapping of biological distributions to microscale physical flows. It is proposed that the multiple imaging wavelengths will allow a degree of taxonomic discrimination among fluorescent particles, while the side-scattered light will provide images of all particles that scatter the excitation irradiance including most of the phytoplankton appearing in the fluorescence images, as well as zooplankton. The 3D PIV-multispectral imaging fluorometer will be mounted on a free-falling platform with a suite of auxiliary instruments and a water sampler for image ground-truthing that has already been constructed and shown to provide undisturbed views of plankton in the water column. Images will be captured in a plane extending below the platform to obtain distributions unaffected by the platform. Shipboard epifluorescence microscopy will be used to identify dominant organisms in the images.Relating microscale distributions of plankton to microscale physical dynamics in the ocean has been largely limited by a lack of technology. The combination of multiple-wavelength imaging and PIV will allow in situ investigations of fluorescent particle/bulk fluorescence relationships, microscale distributions of plankton in relation to microscale flows, spatial relationships of grazers and primary producers, and physical mechanisms underlying fine- and microscale biological patchiness. This instrument is an enhancement of an existing 2D imaging fluorometer developed by the PIs that has only one sensing wavelength, and does not gather simultaneous physical data on the same scales as the fluorescence images.