Collaborative Research: Development of a Combined in Situ Particle Imaging Velocimeter /Fluorescence Imaging System

合作研究：原位粒子成像测速仪/荧光成像组合系统的开发

• 批准号: 0220379
• 负责人: Jules Jaffe
• 金额: --
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0220379&HistoricalAwards=false
• 关键词: Collaborative; Research; Development; Combined; Situ

The PIs propose to build a new system that will image multiple wavelengths of fluoresced and scattered light and perform stereo particle image velocimetry (3D PIV) measurements. These data will enable maps of fluid flow and derived quantities such as strain rate, vorticity, and the dissipation rate of turbulent kinetic energy to be produced. Measurements will be made on identical scales (20 x 20 x 0.6 cm with 100 um resolution), nearly simultaneously, allowing in situ 3D mapping of biological distributions to microscale physical flows. It is proposed that the multiple imaging wavelengths will allow a degree of taxonomic discrimination among fluorescent particles, while the side-scattered light will provide images of all particles that scatter the excitation irradiance including most of the phytoplankton appearing in the fluorescence images, as well as zooplankton. The 3D PIV-multispectral imaging fluorometer will be mounted on a free-falling platform with a suite of auxiliary instruments and a water sampler for image ground-truthing that has already been constructed and shown to provide undisturbed views of plankton in the water column. Images will be captured in a plane extending below the platform to obtain distributions unaffected by the platform. Shipboard epifluorescence microscopy will be used to identify dominant organisms in the images.Relating microscale distributions of plankton to microscale physical dynamics in the ocean has been largely limited by a lack of technology. The combination of multiple-wavelength imaging and PIV will allow in situ investigations of fluorescent particle/bulk fluorescence relationships, microscale distributions of plankton in relation to microscale flows, spatial relationships of grazers and primary producers, and physical mechanisms underlying fine- and microscale biological patchiness. This instrument is an enhancement of an existing 2D imaging fluorometer developed by the PIs that has only one sensing wavelength, and does not gather simultaneous physical data on the same scales as the fluorescence images.

PI 提议建立一个新系统，对多个波长的荧光和散射光进行成像，并执行立体粒子图像测速 (3D PIV) 测量。这些数据将能够生成流体流动图和导出量，例如应变率、涡度和湍流动能的耗散率。测量几乎同时在相同的尺度（20 x 20 x 0.6 cm，分辨率为 100 um）上进行，从而可以将生物分布原位 3D 映射到微尺度物理流。据建议，多个成像波长将允许荧光颗粒之间进行一定程度的分类区分，而侧向散射光将提供散射激发辐照度的所有颗粒的图像，包括荧光图像中出现的大多数浮游植物以及浮游动物。 3D PIV 多光谱成像荧光计将安装在一个自由落体平台上，该平台配有一套辅助仪器和一个用于图像地面实况的水采样器，该采样器已经构建并显示可以提供水柱中浮游生物的不受干扰的视图。图像将在平台下方延伸的平面中捕获，以获得不受平台影响的分布。船上落射荧光显微镜将用于识别图像中的优势生物。由于缺乏技术，将浮游生物的微观分布与海洋中的微观物理动力学联系起来在很大程度上受到限制。多波长成像和 PIV 的结合将允许对荧光颗粒/块体荧光关系、浮游生物与微尺度流的微尺度分布、食草动物和初级生产者的空间关系以及精细和微尺度生物斑块背后的物理机制进行原位研究。该仪器是 PI 开发的现有 2D 成像荧光计的增强版，它只有一个传感波长，并且不会在与荧光图像相同的尺度上同时收集物理数据。