QSB: Development of an Integrated Biological and Computational Approach to Decipher Transcriptional Regulatory Networks in Saccharromyces Cerevisiae

该项目的长期目标是开发一种综合的生物学和计算方法来破译基因调控网络。该项目结合了基于发现的方法来识别和表征新的调节网络，并采用假设驱动的方法，其中将检查半乳糖代谢中两个重要调节因子的转录控制。该项目的具体目标是：(1)暂时描述全球对半乳糖的遗传和蛋白质组反应；(2)在全球范围内确定对半乳糖利用重要的转录网络要素；(3)为转录网络的整合、建模和预测开发先进的计算框架；(4)对这些研究预测的网络模型进行实验检验。

The long range goal of this project is to develop an integrated biological and computational approach to decipher gene regulatory networks. This project combines discovery-based approaches to identify and characterize new regulatory networks with hypothesis-driven approaches in which transcriptional control by two regulatory factors important in galactose metabolism will be examined. The specific aims of this project are: (1) to temporally profile the global genetic and proteomic response to galactose; (2) to globally identify the elements of transcriptional networks important for galactose utilization; (3) to develop an advanced computational framework for integration, modeling, and prediction of transcriptional networks; and (4) to experimentally test the networks models predicted from these studies.